Anti-15 Lipoxygenase 1/ALOX15 Antibody Picoband® Fluoro647 Conjugated

Arachidonate 15-lipoxygenase, also known as 15 LOX or LOG15, is an enzyme that in humans is encoded by the ALOX15 gene. This gene is mapped to 17p13.2. It is found that ALOX15 is a mutator gene. ALOX15 gene product is implicated in antiinflammation, membrane remodeling, and cancer development/metastasis. Loss of the TP53 gene, or gain-of-function activities resulting from the expression of its mutant forms regulates ALOX15 promoter activity in human and in mouse, albeit in ionally opposite manners.

Arachidonate 15-lipoxygenase;15-LOX;15-LOX-1;1.13.11.33;12/15-lipoxygenase; Arachidonate 12-lipoxygenase, leukocyte-type;12-LOX;1.13.11.31; Arachidonate omega-6 lipoxygenase; ALOX15; LOG15

E.coli-derived human ALOX15 recombinant protein (Position: G2-P337) . Human ALOX15 shares 72% and 73% amino acid (aa) sequences identity with mouse and rat ALOX15, respectively.

Atherosclerosis, Cancer, Cardiovascular, Fatty Acid Oxidation, Fatty Acids, Growth Factors/Hormones, Heart Disease, Lipid and Lipoprotein Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Redox Metabolism, Signal Transduction, Tumor Biomarkers

Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Converts arachidonic acid into 12- hydroperoxyeicosatetraenoic acid/12-HPETE and 15- hydroperoxyeicosatetraenoic acid/15-HPETE. Also converts linoleic acid to 13-hydroperoxyoctadecadienoic acid. May also act on (12S) - hydroperoxyeicosatetraenoic acid/ (12S) -HPETE to produce hepoxilin A3. Probably plays an important role in the immune and inflammatory responses. Through the oxygenation of membrane-bound phosphatidylethanolamine in macrophages may favor clearance of apoptotic cells during inflammation by resident macrophages and prevent an autoimmune response associated with the clearance of apoptotic cells by inflammatory monocytes. In parallel, may regulate actin polymerization which is crucial for several biological processes, including macrophage function. May also regulate macrophage function through regulation of the peroxisome proliferator activated receptor signaling pathway. Finally, it is also involved in the cellular response to IL13/interleukin-13. In addition to its role in the immune and inflammatory responses, may play a role in epithelial wound healing in the cornea maybe through production of lipoxin A4. May also play a role in endoplasmic reticulum stress response and the regulation of bone mass. .

1. Funk CD, Funk LB, FitzGerald GA, Samuelsson B (May 1992) .Characterization of human 12-lipoxygenase genes.Proceedings of the National Academy of Sciences of the United States of America 89 (9) : 3962–6. 2. Kelavkar, U. P., Badr, K. F. Effects of mutant p53 expression on human 15-lipoxygenase-promoter activity and murine 12/15-lipoxygenase gene expression: evidence that 15-lipoxygenase is a mutator gene. Proc. Nat. Acad. Sci. 96: 4378-4383, 1999.

Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein. Lipid droplet. Predominantly cytosolic; becomes enriched at membranes upon calcium binding. Translocates from the cytosol to the plasma membrane when stimulated by IL13/interleukin-13 and in macrophages binding apoptotic cells.