Anti-Wnt5a Antibody Picoband®

Protein Wnt-5a is a protein that in humans is encoded by the WNT5A gene. This gene is a member of the WNT gene family. The WNTs comprise a large class of secreted proteins that control essential developmental processes such as embryonic patterning, cell growth, migration, and differentiation. It is mapped to 3p14.3. WNT5A is highly expressed in the dermal papilla of depilated skin. This protein plays an essential role in regulating developmental pathways during embryogenesis. And this protein may also play a role in oncogenesis. WNT5A and DVL can promote axon differentiation mediated by the PAR3-PAR6-aPKC complex.

E.coli-derived human Wnt5a recombinant protein (Position: D184-D303) . Human Wnt5a shares 100% amino acid (aa) sequence identity with both mouse and rat Wnt5a.

Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells (at protein level) . .

Cancer, Neural Signal Transduction, Neurology Process, Neuroscience, Oncoproteins/Suppressors, Secreted, Signaling Pathways, Stem Cells, Tumor Suppressors

Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes. .

1. Entrez Gene: WNT5A wingless-type MMTV integration site family, member 5A 2. Mikels, A. J., Nusse, R. Purified Wnt-5a protein activates or inhibits beta-catenin-TCF signaling depending on receptor context. PLoS Biol. 4: e115, 2006. 3. Zhang, X., Zhu, J., Yang, G.-Y., Wang, Q.-J., Qian, L., Chen, Y.-M., Chen, F., Tao, Y., Hu, H.-S., Wang, T., Luo, Z.-G. Dishevelled promotes axon differentiation by regulating atypical protein kinase C. Nature Cell Biol. 9: 743-754, 2007.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.