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Anti-GM-CSF/CSF2 Antibody Picoband® Fluoro488 Conjugated

Product Specifications

Background

GM-CSF, Granulocyte-macrophage colony-stimulating factor, is a protein secreted by macrophages, T cells, mast cells, endothelial cells, and fibroblasts. By fluorescence in situ hybridization, the GM-CSF gene is mapped to 5q31.1. GM-CSF is a cytokine that functions as a white blood cell growth factor. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. GM-CSF is an essential regulator of neutrophil function.

Synonyms

Granulocyte-macrophage colony-stimulating factor; GM-CSF; Colony-stimulating factor; CSF; Csf2; Csfgm

Gene Name

CSF2

Gene ID

12981

UniProt

P01587

Host

Rabbit

Reactivity

Mouse

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived mouse GM-CSF recombinant protein (Position: A18-K141) . Mouse GM-CSF shares 54% and 70% amino acid (aa) sequences identity with human and rat GM-CSF, respectively.

Clonality

Polyclonal

Tissue Specificity

Synthesized exclusively by adipocytes and secreted into plasma.

Applications

Flow Cytometry

Field of Research

Cytokines, Immunology, Innate Immunity

Purification

Immunogen affinity purified.

Form

Liquid

Function

Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

References & Citations

1. Le Beau, M. M., Espinosa, R., III, Neuman, W. L., Stock, W., Roulston, D., Larson, R. A., Keinanen, M., Westbrook, C. A.Cytogenetic and molecular delineation of the smallest commonly deleted region of chromosome 5 in malignant myeloid diseases.Proc. Nat. Acad. Sci. 90: 5484-5488, 1993. 2. Park, B. K., Zhang, H., Zeng, Q., Dai, J., Keller, E. T., Giordano, T., Gu, K., Shah, V., Pei, L., Zarbo, R. J., McCauley, L., Shi, S., Chen, S., Wang, C.-Y.NF-kappa-B in breast cancer cells promotes osteolytic bone metastasis by inducing osteoclastogenesis via GM-CSF.Nature Med. 13: 62-69, 2007. 3. Uchida, K., Beck, D. C., Yamamoto, T., Berclaz, P.-Y., Abe, S., Staudt, M. K., Carey, B. C., Filippi, M.-D., Wert, S. E., Denson, L. A., Puchalski, J. T., Hauck, D. M., Trapnell, B. C.GM-CSF autoantibodies and neutrophil dysfunction in pulmonary alveolar proteinosis.New Eng. J. Med. 356: 567-579, 2007.

Storage Conditions

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Calculated Molecular Weight

16091 MW

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Granulocyte-macrophage colony-stimulating factor

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the GM-CSF family.

Protein Name

Granulocyte-macrophage colony-stimulating factor

Isotype

Rabbit IgG

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

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