What sizes are available for Anti-E3 SUMO-protein ligase PIAS4 Antibody Picoband® Fluoro488 Conjugated?

Anti-E3 SUMO-protein ligase PIAS4 Antibody Picoband® Fluoro488 Conjugated is available in the following size(s): 100 µg/Vial.

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.

Anti-E3 SUMO-protein ligase PIAS4 Antibody Picoband® Fluoro488 Conjugated

Q: What is the catalog number for Anti-E3 SUMO-protein ligase PIAS4 Antibody Picoband® Fluoro488 Conjugated?

The catalog number for Anti-E3 SUMO-protein ligase PIAS4 Antibody Picoband® Fluoro488 Conjugated is PA2215-Fluoro488.

Product Specifications

Background

E3 SUMO-protein ligase PIAS4, also known as protein inhibitor of activated STAT protein 4 (PIAS4) or protein inhibitor of activated STAT protein gamma (PIASg or PIASy) is an enzyme that in humans is encoded by the PIAS4 gene. This gene is mapped to 19p13.3. This gene plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. It functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. This gene involved in gene silencing.

Synonyms

E3 SUMO-protein ligase PIAS4;6.3.2.-; PIASy; Protein inhibitor of activated STAT protein 4; Protein inhibitor of activated STAT protein gamma; PIAS-gamma; PIAS4; PIASG

Gene Name

PIAS4

Gene ID

51588

UniProt

Q8N2W9

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human PIAS4, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Tissue Specificity

Highly expressed in testis and, at lower levels, in spleen, prostate, ovary, colon and peripheral blood leukocytes. .

Applications

Flow Cytometry

Field of Research

Chromatin Binding Proteins, Chromatin Modifying Enzymes, Co-Factors, DNA/RNA Binding, Epigenetics and Nuclear Signaling, Nuclear Signaling Pathways, Transcription, Ubiquitin & Ubiquitin Like Modifiers

Purification

Immunogen affinity purified.

Form

Liquid

Function

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Mediates sumoylation of CEBPA, PARK7, HERC2, MYB, TCF4 and RNF168. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. .

References & Citations

1. Bischof, O., Schwamborn, K., Martin, N., Werner, A., Sustmann, C., Grosschedl, R., Dejean, A. The E3 SUMO ligase PIASy is a regulator of cellular senescence and apoptosis. Molec. Cell 22: 783-794, 2006. 2. Galanty, Y., Belotserkovskaya, R., Coates, J., Polo, S., Miller, K. M., Jackson, S. P. Mammalian SUMO E3-ligases PIAS1 and PIAS4 promote responses to DNA double-strand breaks. Nature 462: 935-939, 2009. 3. Sachdev, S., Bruhn, L., Sieber, H., Pichler, A., Melchior, F., Grosschedl, R. PIASy, a nuclear matrix-associated SUMO E3 ligase, represses LEF1 activity by sequestration into nuclear bodies. Genes Dev. 15: 3088-3103, 2001.

Storage Conditions

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Calculated Molecular Weight

56504 MW

Specificity

No cross reactivity with other proteins.

Applications Notes

Gene Name Synonym

E3 SUMO-protein ligase PIAS4

Subcellular Location

Nucleus, PML body . Colocalizes with SUMO1 and TCF7L2/TCF4 and LEF1 in a subset of PML (promyelocytic leukemia) nuclear bodies.

Sequence Similarities

Belongs to the PIAS family.

Protein Name

E3 SUMO-protein ligase PIAS4

Isotype

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

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