Anti-Leupaxin/LPXN Antibody Picoband® Fluoro647 Conjugated

Leupaxin is a protein that in humans is encoded by the LPXN gene. This gene is mapped to 11q12.1. The product encoded by this gene is preferentially expressed in hematopoietic cells and belongs to the paxillin protein family. Similar to other members of this focal-adhesion-associated adaptor-protein family, it has four leucine-rich LD-motifs in the N-terminus and four LIM domains in the C-terminus. It may function in cell type-specific signaling by associating with PYK2, a member of focal adhesion kinase family. As a substrate for a tyrosine kinase in lymphoid cells, this protein may also function in, and be regulated by, tyrosine kinase activity.

A synthetic peptide corresponding to a sequence at the N-terminus of human Leupaxin, different from the related rat sequence by two amino acids, and from the related mouse sequence by three amino acids.

Macrophages, monocytes and osteoclasts (at protein level) . Strongly expressed in cells and tissues of hematopoietic origin. Highest expression in lymphoid tissues such as spleen, lymph node, thymus and appendix and in the vascular smooth muscle. Lower levels in bone marrow and fetal liver. Also expressed in peripheral blood lymphocytes and a number of hematopoietic cell lines. Very low levels found in epithelial cell lines. Expressed in prostate cancer (PCa) cells and its expression intensity is ly linked to PCa progression. .

Adaptive Immunity, Apoptosis, Cancer, Cell Biology, Cell Death, DNA/RNA, Epigenetics and Nuclear Signaling, Immunology, Non-CD, Nucleus, RNA Processing, T Cells

Transcriptional coactivator for androgen receptor (AR) and serum response factor (SRF) . Contributes to the regulation of cell adhesion, spreading and cell migration and acts as a negative regulator in integrin-mediated cell adhesion events. Suppresses the integrin-induced tyrosine phosphorylation of paxillin (PXN) . May play a critical role as an adapter protein in the formation of the adhesion zone in osteoclasts. Negatively regulates B-cell antigen receptor (BCR) signaling. .

1. Lipsky, B. P., Beals, C. R., Staunton, D. E. Leupaxin is a novel LIM domain protein that forms a complex with PYK2. J. Biol. Chem. 273: 11709-11713, 1998. 2. Entrez Gene: LPXN leupaxin.

Cytoplasm. Cell junction, focal adhesion. Nucleus. Cytoplasm, perinuclear region . Cell projection, podosome. Cell membrane. Shuttles between the cytoplasm and nucleus. Recruited to the cell membrane following B- cell antigen receptor (BCR) cross-linking in B-cells. Enhanced focal adhesion kinase activity (PTK2/FAK) attenuates its nuclear accumulation and limits its ability to enhance serum response factor (SRF) -dependent gene transcription. Targeting to focal adhesions is essential for its tyrosine phosphorylation in response to bombesin.