Anti-HIF3/HIF3A Antibody Picoband® Fluoro488 Conjugated

Hypoxia-inducible factor 3 alpha, also called MOP7, is a protein that in humans is encoded by the HIF3A gene. HIF3A is mapped to 19q13.32. This gene involved in adaptive response to hypoxia. HIF3A suppresses hypoxia-inducible expression of HIF1A and EPAS1. It binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. This gene functions as an inhibitor of angiogenesis in the cornea.

Hypoxia-inducible factor 3-alpha ; HIF-3-alpha ; HIF3-alpha; Basic-helix-loop-helix-PAS protein MOP7; Class E basic helix-loop-helix protein 17; bHLHe17; HIF3-alpha-1; Inhibitory PAS domain protein; IPAS; Member of PAS protein 7; PAS domain-containing protein 7; HIF3A ; BHLHE17, MOP7, PASD7

A synthetic peptide corresponding to a sequence at the C-terminus of human HIF3, different from the related rat and mouse sequences by five amino acids.

Expressed in vascular cells (at protein level) (PubMed:21069422) . Expressed in kidney (PubMed:11573933, PubMed:19694616) . Expressed in lung epithelial cells (PubMed:16775626) . Expressed in endothelial cells (venous and arterial cells from umbilical cord and aortic endothelial cells) and in vascular smooth muscle cells (aorta) (PubMed:21069422) . Strongly expressed in the heart, placenta, and skeletal muscle, whereas a weak expression profile was found in the lung, liver, and kidney (PubMed:12538644) . Expressed weakly in cell renal cell carcinoma (CC-RCC) compared to normal renal cells (PubMed:16126907) . Expression is down-regulated in numerous kidney tumor cells compared to non tumor kidney tissues (PubMed:16126907) . Isoform 2 is expressed in heart, placenta, lung, liver, skeletal muscle and pancreas and in numerous cancer cell lines (PubMed:20416395) . Isoform 3 and isoform 4 are weakly expressed in heart, placenta, lung, liver, skeletal muscle and pancreas (PubMed:20416395) . Isoform 4 is expressed in fetal tissues, such as heart, brain, thymus, lung, liver, skeletal kidney and spleen (PubMed:20416395) . Isoform 3 is weakly expressed in fetal tissues, such as liver and kidney (PubMed:20416395) . .

Acts as a transcriptional regulator in adaptive response to low oxygen tension. Acts as a regulator of hypoxia-inducible gene expression (PubMed:11573933, PubMed:16126907, PubMed:19694616, PubMed:20416395, PubMed:21069422) . Functions as an inhibitor of angiogenesis in hypoxic cells of the cornea. Plays a role in the development of the cardiorespiratory system. May also be involved in apoptosis (By similarity) . .

1. Hara, S., Hamada, J., Kobayashi, C., Kondo, Y., Imura, N. Expression and characterization of hypoxia-inducible factor (HIF) -3-alpha in human kidney: suppression of HIF-mediated gene expression by HIF-3-alpha. Biochem. Biophys. Res. Commun. 287: 808-813, 2001. 2. Maynard, M. A., Qi, H., Chung, J., Lee, E. H. L., Kondo, Y., Hara, S., Conaway, R. C., Conaway, J. W., Ohh, M. Multiple splice variants of the human HIF-3-alpha locus are targets of the von Hippel-Lindau E3 ubiquitin ligase complex. J. Biol. Chem. 278: 11032-11040, 2003.

Nucleus . Cytoplasm . Nucleus speckle . Mitochondrion . In the nuclei of all periportal and perivenous hepatocytes. In the distal perivenous zone, detected in the cytoplasm of the hepatocytes. Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner. Colocalizes with BAD in the cytoplasm. Colocalizes with EPAS1 and HIF1A in the nucleus and speckles (By similarity) . Localized in the cytoplasm and nuclei under normoxia, but increased in the nucleus under hypoxic conditions (PubMed:19694616) . Colocalized with HIF1A in kidney tumors (PubMed:19694616) . .