Anti-Gelsolin/GSN Antibody Picoband®

Gelsolin also known as GNS is an actin-binding protein that is a key regulator of actin filament assembly and disassembly. Gelsolin is one of the most potent members of the actin-severing gelsolin/villin superfamily. The gene was assigned to human chromosome 9q33.2. Gelsolin is also known as brevin, or actin-depolymerizing factor; it is the principal intracellular and extracellular actin-severing protein. Gelsolin and Gc protein together constitute the extracellular actin-scavenger system which prevents the toxic effects of actin release into the extracellular space under circumstances of cell necrosis. Gelsolin may have therapeutic potential as a mucolytic agent in CF patients. The antiapoptotic activity of gelsolin seems to prevent a step leading to cytochrome c release from the mitochondria into the cytosol.

A synthetic peptide corresponding to a sequence at the C-terminus of human Gelsolin, identical to the related rat sequence, and different from the related mouse sequence by one amino acid.

Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping) . It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis. .

1. Kamada, S., Kusano, H., Fujita, H., Ohtsu, M., Koya, R. C., Kuzumaki, N., Tsujimoto, Y. A cloning method for caspase substrates that uses the yeast two-hybrid system: cloning of the antiapoptotic gene gelsolin. Proc. Nat. Acad. Sci. 95: 8532-8537, 1998. 2. Kim, J., Lee, J. E., Heynen-Genel, S., Suyama, E., Ono, K., Lee, K., Ideker, T., Aza-Blanc, P., Gleeson, J. G. Functional genomic screen for modulators of ciliogenesis and cilium length. Nature 464: 1048-1051, 2010. 3. Vasconcellos, C. A., Allen, P. G., Wohl, M. E., Drazen, J. M., Janmey, P. A., Stossel, T. P. Reduction in viscosity of cystic fibrosis sputum in vitro by gelsolin. Science 263: 969-971, 1994.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.