Anti-RGS3 Antibody Picoband® Fluoro594 Conjugated
Product Specifications
Background
RGS3 (Regulator of G Protein Signaling 3), is a protein that in humans is encoded by the RGS3 gene. Chatterjee et al. (1997) studied RGS3, the largest member of the RGS family then identified. Mouse Pdz-Rgs3 can mediate signaling from the ephrin-B cytoplasmic tail. By radiation hybrid mapping, Chatterjee et al. (1997) assigned the RGS3 gene to chromosome 9q31-q33. Lu et al. (2001) showed that Sdf1, a chemokine with a G protein-coupled receptor, and Bdnf are chemoattractants for cerebellar granule cells, and that Sdf1 chemoattraction is selectively inhibited by soluble ephrin-B receptor. This inhibition could be blocked by a truncated Pdz-Rgs3 protein lacking the RGS domain.
Synonyms
Regulator of G-protein signaling 3; RGS3; C2PA; Rgs3
Gene Name
Rgs3
Gene ID
50780
UniProt
Q9DC04
Host
Rabbit
Reactivity
Rat, Mouse, Human
Cross Reactivity
No cross-reactivity with other proteins
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse RGS3, identical to the related rat sequence, and different from the related human sequence by two amino acids.
Clonality
Polyclonal
Tissue Specificity
Detected in embryos from E8.5-16.5 in cortical ventricular zone, dorsal root ganglia and cerebellar primordia. Isoform 3 is detected in testis and in spermatocytes from newborn mice. Levels increase and reach a maximum after 21 days; after this they decrease again. Long isoforms are widely expressed. .
Applications
Flow Cytometry
Field of Research
G Protein Signaling, Regulators, Signal Transduction, Signaling Pathway, Small G Proteins
Purification
Immunogen affinity purified.
Form
Liquid
Function
Down-regulates signaling from heterotrimeric G-proteins by increasing the GTPase activity of the alpha subunits, thereby driving them into their inactive GDP-bound form. Down-regulates G- protein-mediated release of inositol phosphates and activation of MAP kinases. .
References & Citations
1. Chatterjee, T. K., Eapen, A., Kanis, A. B., Fisher, R. A. Genomic organization, 5-prime-flanking region, and chromosomal localization of the human RGS3 gene. Genomics 45: 429-433, 1997. 2. Druey, K. M., Blumer, K. J., Kang, V. H., Kehrl, J. H. Inhibition of G-protein-mediated MAP kinase activation by a new mammalian gene family. Nature 379: 742-746, 1996. 3. Lu, Q., Sun, E. E., Klein, R. S., Flanagan, J. G. Ephrin-B reverse signaling is mediated by a novel PDZ-RGS protein and selectively inhibits G protein-coupled chemoattraction. Cell 105: 69-79, 2001.
Storage Conditions
At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Calculated Molecular Weight
106219 MW
Specificity
No cross reactivity with other proteins.
Applications Notes
6
Gene Name Synonym
Regulator of G-protein signaling 3
Subcellular Location
Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. The long isoforms are cytoplasmic.
Sequence Similarities
Contains 1 PDZ (DHR) domain.
Protein Name
Regulator of G-protein signaling 3
Isotype
Rabbit IgG
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
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