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Anti-Selenocysteine lyase SCLY Antibody Picoband®

Boster Bio Anti-Selenocysteine lyase SCLY Antibody catalog # PA1995. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

SCLY (Selenocysteine Lyase), is an enzyme that catalyzes the chemical reaction. This enzyme belongs to the family of lyases, specifically the class of carbon-sulfur lyases. The International Radiation Hybrid Mapping Consortium mapped the human SCLY gene to chromosome 2. Mihara et al. (2000) showed that mouse Scly catalyzed the conversion of L-selenocysteine to L-alanine. Scly activity required pyridoxal 5-prime phosphate, was specific to L-selenocysteine, and showed maximum reactivity at pH 9.0.

Synonyms

Selenocysteine lyase; hSCL;4.4.1.16; SCLY; SCL

Gene Name

SCLY

Gene ID

51540

UniProt

Q96I15

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human SCLY.

Clonality

Polyclonal

Tissue Specificity

Expressed in many tissues, with the highest level in heart and skeletal muscle, intermediate levels in brain, kidney, pancreas and liver, and low levels in placenta and lung. .

Applications

WB

Field of Research

Amino Acid Metabolism, Amino Acids, Cofactors, Vitamins/Minerals, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction, Vitamins/Minerals

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Catalyzes the decomposition of L-selenocysteine to L- alanine and elemental selenium. .

References & Citations

1. Mihara, H., Kurihara, T., Watanabe, T., Yoshimura, T., Esaki, N. cDNA cloning, purification, and characterization of mouse liver selenocysteine lyase: candidate for selenium delivery protein in selenoprotein synthesis. J. Biol. Chem. 275: 6195-6200, 2000.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

48149 MW

Observed Molecular Weight

85-91 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Selenocysteine lyase

Subcellular Location

Cytoplasm, cytosol .

Sequence Similarities

Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.

Protein Name

Selenocysteine lyase

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

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