Anti-TRPV3 Antibody Picoband®

TRPV3 (Transient Receptor Potential Cation Channel Subfamily V Member 3), also known as VRL3, is a human gene encoding the protein of the same name. The TRPV3 protein belongs to a family of nonselective cation channels that function in a variety of processes, including temperature sensation and vasoregulation. Peier et al. (2002) localized the TRPV3 gene to a BAC clone mapped to chromosome 17p13. They mapped the mouse gene to chromosome 11B4. Peier et al. (2002) stably expressed mouse Trpv3 in Chinese hamster ovary cells and assayed electrophysiologic activity by whole cell voltage-clamp techniques. They determined that Trpv3 is a cation-permeable channel activated by warm and hot temperatures. Xu et al. (2002) showed that increasing temperature from approximately 22 to 40 degrees Celsius in mammalian cells transfected with human TRPV3 elevated intracellular calcium by activating a nonselective cationic conductance.

A synthetic peptide corresponding to a sequence at the C-terminus of human TRPV3, different from the related rat and mouse sequences by four amino acids.

Abundantly expressed in CNS. Widely expressed at low levels. Detected in dorsal root ganglion (at protein level) . Expressed in the keratinocyte layers of the outer root sheath and, to lesser extent, to the matrix of the hair follicles (at protein level) . .

Putative receptor-activated non-selective calcium permeant cation channel. It is activated by innocuous (warm) temperatures and shows an increased response at noxious temperatures greater than 39 degrees Celsius. Activation exhibits an outward rectification. May associate with TRPV1 and may modulate its activity. Is a negative regulator of hair growth and cycling: TRPV3-coupled signaling suppresses keratinocyte proliferation in hair follicles and induces apoptosis and premature hair follicle regression (catagen) . .

1. Peier, A. M., Reeve, A. J., Andersson, D. A., Moqrich, A., Earley, T. J., Hergarden, A. C., Story, G. M., Colley, S., Hogenesch, J. B., McIntyre, P., Bevan, S., Patapoutian, A. A heat-sensitive TRP channel expressed in keratinocytes. Science 296: 2046-2049, 2002. 2. Xu, H., Ramsey, I. S., Kotecha, S. A., Moran, M. M., Chong, J. A., Lawson, D., Ge, P., Lilly, J., Silos-Santiago, I., Xie, Y., DiStefano, P. S., Curtis, R., Clapham, D. E. TRPV3 is a calcium-permeable temperature-sensitive cation channel. Nature 418: 181-186, 2002.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.