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Anti-Zinc finger protein GLI2 Gli2 Antibody Picoband® Fluoro488 Conjugated

Q: What is the catalog number for Anti-Zinc finger protein GLI2 Gli2 Antibody Picoband® Fluoro488 Conjugated?

The catalog number for Anti-Zinc finger protein GLI2 Gli2 Antibody Picoband® Fluoro488 Conjugated is PA1941-Fluoro488.

Selected Variant

PA1941-Fluoro488

Size: 100 µg/Vial

Product Specifications

Background

GLI2 (Gli-Kruppel Family Member 2), also called ONCOGENE GLI2, is a protein that in humans is encoded by the GLI2 gene. Sequencing of GLI cDNA clones showed the presence of 5 tandem zinc fingers connected by histidine-cysteine links, which indicated that the gene belongs to the family of zinc finger genes related to Kruppel (Kr) . The Drosophila gene Kr is a member of the gap class of segmentation genes; thoracic and anterior abdominal segments fail to form in Kr mutant embryos. By fluorescence in situ hybridization, Matsumoto et al. (1996) refined the assignment of the GLI2 gene to chromosome 2q14. Roessler et al. (2005) showed that GLI2-delta-N exhibited potent transcriptional activity in vivo: overexpression in mouse skin led to the formation of hedgehog-independent epithelial downgrowths resembling basal cell carcinomas, which in humans are associated with constitutive hedgehog signaling.

Synonyms

Zinc finger protein GLI2 ; GLI family zinc finger protein 2 ; Tax helper protein ; GLI2 ; THP

Gene Name

GLI2

Gene ID

2736

UniProt

P10070

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Gli2.

Clonality

Polyclonal

Tissue Specificity

Isoform 1 and isoform 4 are expressed in HTLV- 1-infected T-cell lines (at protein level) . Isoform 1 and isoform 2 are strongly expressed in HTLV-1-infected T-cell lines. Isoform 3 and isoform 4 are weakly expressed in HTLV-1-infected T-cell lines. .

Applications

Flow Cytometry

Field of Research

2339, Domain Families, Epigenetics and Nuclear Signaling, Neural Signal Transduction, Neurology Process, Neuroscience, Nuclear, Signaling Pathways, Stem Cells, Transcription, Zinc Finger

Purification

Immunogen affinity purified.

Form

Liquid

Function

Has a dual function as a transcriptional activator and a repressor of the hedgehog (Hh) pathway. May play a role during embryogenesis. .

References & Citations

1. Bertolacini, C. D. P., Ribeiro-Bicudo, L. A., Petrin, A., Richieri-Costa, A., Murray, J. C. Clinical findings in patients with GLI2 mutations--phenotypic variability. Clin. Genet. 81: 70-75, 2012. 2. Matsumoto, N., Fujimoto, M., Kato, R., Niikawa, N. Assignment of the human GLI2 gene to 2q14 by fluorescence in situ hybridization. Genomics 36: 220-221, 1996. 3. Roessler, E., Ermilov, A. N., Grange, D. K., Wang, A., Grachtchouk, M., Dlugosz, A. A., Muenke, M. A previously unidentified amino-terminal domain regulates transcriptional activity of wild-type and disease-associated human GLI2. Hum. Molec. Genet. 14: 2181-2188, 2005.

Storage Conditions

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Calculated Molecular Weight

167783 MW

Specificity

No cross reactivity with other proteins.

Applications Notes

Gene Name Synonym

Zinc finger protein GLI2

Subcellular Location

Nucleus . Cytoplasm . In keratinocytes, it is sequestered in the cytoplasm by SUFU. In the absence of SUFU, it translocates to the nucleus. .

Sequence Similarities

Belongs to the GLI C2H2-type zinc-finger protein family.

Protein Name

Zinc finger protein GLI2

Isotype

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

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