Anti-Acid-sensing ion channel 3 ASIC3 Antibody Picoband®

ASIC3 (Acid-Sensing Ion Channel3), also known as TESTIS SODIUM CHANNEL 1 (TNAC1) or DORSAL ROOT ACID-SENSING ION CHANNEL (DRASIC), is a protein that in humans is encoded by the ASIC3 gene. ASIC3 belongs to a family of acid-sensing channel proteins that are structurally related to epithelial sodium channel proteins and support acid-activated membrane currents. By radiation hybrid analysis, de Weille et al. (1998) mapped the ACCN3 gene to chromosome 7q35. De Weille et al. (1998) found that human ASIC3 supported an H (+) -gated cation current in COS cells with kinetics similar to those of rat Asic3. Babinski et al. (1999) expressed homomeric human ASIC3 channels in Xenopus oocytes and found that rapid reduction in extracellular pH resulted in a biphasic response characterized by a fast and rapidly desensitizing current followed by a slow and sustained current that returned to baseline only on return to physiologic pH.

Acid-sensing ion channel 3; ASIC3; hASIC3; Amiloride-sensitive cation channel 3; Neuronal amiloride-sensitive cation channel 3; Testis sodium channel 1; hTNaC1; ASIC3; ACCN3, SLNAC1, TNAC1

A synthetic peptide corresponding to a sequence at the N-terminus of human ASIC3, different from the related rat and mouse sequences by two amino acids.

Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Generates a biphasic current with a fast inactivating and a slow sustained phase. In sensory neurons is proposed to mediate the pain induced by acidosis that occurs in ischemic, damaged or inflamed tissue. May be involved in hyperalgesia. May play a role in mechanoreception. Heteromeric channel assembly seems to modulate channel properties. .

1. Babinski, K., Le, K.-T., Seguela, P. Molecular cloning and regional distribution of a human proton receptor subunit with biphasic functional properties. J. Neurochem. 72: 51-57, 1999. 2. Birdsong, W. T., Fierro, L., Williams, F. G., Spelta, V., Naves, L. A., Knowles, M., Marsh-Haffner, J., Adelman, J. P., Almers, W., Elde, R. P., McCleskey, E. W. Sensing muscle ischemia: coincident detection of acid and ATP via interplay of two ion channels. Neuron 68: 739-749, 2010. 3. de Weille, J. R., Bassilana, F., Lazdunski, M., Waldmann, R. Identification, functional expression and chromosomal localisation of a sustained human proton-gated cation channel. FEBS Lett. 433: 257-260, 1998.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Cell membrane ; Multi-pass membrane protein . Cytoplasm . Cell surface expression may be stabilized by interaction with LIN7B and cytoplasmic retention by interaction with DLG4. In part cytoplasmic in cochlea cells (By similarity) . .