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Anti-MEKK3/MAP3K3 Antibody Picoband® Fluoro647 Conjugated

Product Specifications

Background

MAP3K3 (Mitogen-activated protein kinase kinase kinase 3), also known as MAP/ERK KINASE KINASE 3, MEKK3 or MAPKKK3, is an enzyme that in humans is encoded by the MAP3K3 gene. MAP3K3 is contiguously distal to LYK5 on chromosome 17 (Puffenberger et al., 2007) . By yeast 2-hybrid analysis of a mouse T-cell cDNA library, Uhlik et al. (2003) showed that a C-terminal fragment of mouse Osm (CCM2) interacted with Mekk3, which activates p38 in response to sorbitol-induced hyperosmotic conditions. Mekk3 and Osm colocalized in the cytoplasmic compartment of cotransfected cells, and the Mekk3-Osm complex was recruited to Rac1 and cytoskeletal actin-containing membrane ruffles in response to sorbitol treatment. Protein interaction assays showed that Osm interacted ly with the Mekk3 substrate Mkk3 (MAP2K3), with actin, and with both GDP- and GTP-loaded Rac1. Uhlik et al. (2003) concluded that the RAC1-OSM-MEKK3-MKK3 complex is required for regulation of p38 activity in response to osmotic shock.

Synonyms

Mitogen-activated protein kinase kinase kinase 3;2.7.11.25; MAPK/ERK kinase kinase 3; MEK kinase 3; MEKK 3; Map3k3; Mekk3

Gene Name

MAP3K3

Gene ID

26406

UniProt

Q61084

Host

Rabbit

Reactivity

Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of mouse MEKK3, different from the related rat sequence by two amino acids.

Clonality

Polyclonal

Applications

Flow Cytometry

Field of Research

Immunology, Innate Immunity, MAPK Pathway, Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction, TLR Signaling

Purification

Immunogen affinity purified.

Form

Liquid

Function

Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators. .

References & Citations

1. Channavajhala, P. L., Rao, V. R., Spaulding, V., Lin, L. L., Zhang, Y. G. hKSR-2 inhibits MEKK3-activated MAP kinase and NF-kappa-B pathways in inflammation. Biochem. Biophys. Res. Commun. 334: 1214-1218, 2005. 2. Puffenberger, E. G., Strauss, K. A., Ramsey, K. E., Craig, D. W., Stephan, D. A., Robinson, D. L., Hendrickson, C. L., Gottlieb, S., Ramsay, D. A., Siu, V. M., Heuer, G. G., Crino, P. B., Morton, D. H. Polyhydramnios, megalencephaly and symptomatic epilepsy caused by a homozygous 7-kilobase deletion in LYK5. Brain 130: 1929-1941, 2007. 3. Uhlik, M. T., Abell, A. N., Johnson, N. L., Sun, W., Cuevas, B. D., Lobel-Rice, K. E., Horne, E. A., Dell'Acqua, M. L., Johnson, G. L. Rac-MEKK3-MKK3 scaffolding for p38 MAPK activation during hyperosmotic shock. Nature Cell Biol. 5: 1104-1110, 2003.

Storage Conditions

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Calculated Molecular Weight

70776 MW

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Mitogen-activated protein kinase kinase kinase 3

Sequence Similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

Protein Name

Mitogen-activated protein kinase kinase kinase 3

Isotype

Rabbit IgG

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

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