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Anti-beta Actin/ACTB Antibody Picoband® Fluoro488 Conjugated

Product Specifications

Background

Beta-Actin (ACTB) also known as BETA-ACTIN or ACTIN, CYTOPLASMIC, 1,   is one of six different  actin  isoforms which have been identified in humans. This is one of the two nonmuscle  cytoskeletal  actins. Actins are highly conserved proteins  that are involved in cell motility, structure and integrity.  Ueyama et al. (1996)   used fluorescence in situ hybridization to map ACTB to 7p22. By PCR of somatic cell hybrid DNAs, they mapped 4 ACTB pseudogenes to other chromosomes. Karakozova et al. (2006)   found that arginylation of beta-actin regulates cell motility. The majority of Ate1-null cells appeared smaller than wildtype cells and were apparently unable to form a lamella during movement along the substrate.  Karakozova et al. (2006)   concluded that arginylation of beta-actin apparently represents a critical step in the actin N-terminal processing needed for actin functioning in vivo.

Synonyms

Actin, cytoplasmic 1; Beta-actin; Actin, cytoplasmic 1, N-terminally processed; ACTB

Gene Name

ACTB

Gene ID

60

UniProt

P60709

Host

Rabbit

Reactivity

Chicken, Human, Monkey, Mouse, Rat, Zebrafish

Cross Reactivity

No cross reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human beta Actin, identical to the related rat and mouse sequences.

Clonality

Polyclonal

Tissue Specificity

Brain specific. .

Applications

Flow Cytometry

Field of Research

Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction

Purification

The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Form

Liquid

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

References & Citations

1. Gunning, P., Ponte, P., Okayama, H., Engel, J., Blau, H., Kedes, L. Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed. Molec. Cell. Biol. 3: 787-795, 1983. 2. Karakozova, M., Kozak, M., Wong, C. C. L., Bailey, A. O., Yates, J. R, III, Mogilner, A., Zebroski, H., Kashina, A. Arginylation of beta-actin regulates actin cytoskeleton and cell motility. Science 313: 192-196, 2006. 3. Ueyama, H., Inazawa, J., Nishino, H., Ohkubo, I., Miwa, T. FISH localization of human cytoplasmic actin genes ACTB to 7p22 and ACTG1 to 17q25 and characterization of related pseudogenes.Cytogenet. Cell Genet. 74: 221-224, 1996.

Storage Conditions

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Calculated Molecular Weight

41737 MW

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Actin, cytoplasmic 1

Subcellular Location

Cytoplasm, cytoskeleton . Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Sequence Similarities

Belongs to the actin family.

Protein Name

Actin, cytoplasmic 1

Isotype

IgG

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

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