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Anti-Transcription factor Sp4 Antibody Picoband®

Boster Bio Anti-Transcription factor Sp4 Antibody catalog # PA1865. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

SP4 (Sp4 transcription factor), also known as Transcription factor Sp4, MGC130008, MGC130009, SPR-1, HF1B,   is a  protein  that in humans is encoded by the  SP4  gene. It is a multigene family of SP proteins. Northern blot analysis that SP4 is abundantly expressed as a 7.5-kb message in brain, but barely detectable in other human cell lines. SP4 is an activator protein like SP1. However, in contrast to SP1, SP4 is not able to act synergistically through adjacent binding sites.

Synonyms

Transcription factor Sp4; SPR-1; SP4

Gene Name

SP4

Gene ID

6671

UniProt

Q02446

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human SP4, identical to the related rat sequence, and different from the related mouse sequence by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Abundant in brain.

Applications

WB

Field of Research

Cancer, Cell Biology, Cell Cycle, Epigenetics and Nuclear Signaling

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Binds to GT and GC boxes promoters elements. Probable transcriptional activator.

References & Citations

1. Hagen, G., Muller, S., Beato, M., Suske, G. Cloning by recognition site screening of two novel GT box binding proteins: a family of Sp1 related genes. Nucleic Acids Res. 20: 5519-5525, 1992. 2. Hagen, G., Dennig, J., Preiss, M., Beato, M., Suske, G.Functional analyses of the transcription factor Sp4 reveal properties distinct from Sp1 and Sp3. J. Biol. Chem. 270: 24989-24994, 1995.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

81985 MW

Observed Molecular Weight

82 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Transcription factor Sp4

Subcellular Location

Nucleus.

Sequence Similarities

Belongs to the Sp1 C2H2-type zinc-finger protein family.

Protein Name

Transcription factor Sp4

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

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