Anti-Orai1 Antibody Picoband® Fluoro488 Conjugated

ORAI1 (ORAI calcium release-activated calcium modulator 1), also known as CRACM1, TMEM142A, Calcium release-activated calcium channel protein 1, Protein orai-1, Transmembrane protein 142A, FLJ14466, is a calcium selective  ion channel  that in humans is encoded by the  ORAI1  gene. Orai1 channels play an important role in the activation of T-lymphocytes. The loss of function mutation of Orai1 causes  severe combined immunodeficiency (SCID) in humans. The mammalian orai family has two additional homologs, orai2 and orai3. Orai proteins share no homology with any other ion channel family of any other known proteins. They have 4  transmembrane  domains and form tetramers.  Prakriya et al. (2006) showed that ORAI1 is a PM protein, and that CRAC channel function is sensitive to mutation of 2 conserved acidic residues in the transmembrane segments. Glu106-to-asp (E106D) and glu190-to-gln (E190Q) substitutions in transmembrane helices 1 and 3, respectively, diminished calcium ion influx, increased current carried by monovalent cations, and rendered the channel permeable to cesium ion.  Prakriya et al. (2006)   showed that ORAI1 is a PM protein, and that CRAC channel function is sensitive to mutation of 2 conserved acidic residues in the transmembrane segments.

A synthetic peptide corresponding to a sequence at the C-terminus of human Orai1, different from the related rat and mouse sequences by two amino acids.

Ca (2+) release-activated Ca (2+) (CRAC) channel subunit which mediates Ca (2+) influx following depletion of intracellular Ca (2+) stores and channel activation by the Ca (2+) sensor, STIM1. CRAC channels are the main pathway for Ca (2+) influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT. .

1. Prakriya, M., Feske, S., Gwack, Y., Srikanth, S., Rao, A., Hogan, P. G. Orai1 is an essential pore subunit of the CRAC channel. Nature 443: 230-233, 2006.

Cell membrane ; Multi-pass membrane protein . Cytoplasmic vesicle, autophagosome . Isoform beta is more mobile in the plasma membrane. Colocalizes with UBQLN1 in the autophagosome. .