Anti-STAT5a Antibody Picoband®

STAT5 (Signal transducer and activator of transcription 5) also known as STAT5A, MGF, is a protein that serve the dual function of signal transducers and activators of transcription in cells exposed to signaling polypeptides. Hou et al. (1995)   cloned the human  STAT5  cDNA from an umbilical vein endothelial cell library and found that it encodes a 794-amino acid polypeptide with a predicted mass of approximately 90.5 kD. To analyze the possible role of  STAT5  in human GH-induced proliferation,   Friedrichsen et al. (2001)   expressed a dominant-negative  STAT5  mutant, STAT5A-delta-749, in INS-1 cells under the control of a doxycycline-inducible promoter by stable transfection.  Chromatin immunoprecipitation analysis confirmed binding of  STAT5  to the HIF2A promoter 344 bp upstream of the HIF2A start site.  STAT5  overexpression independently resulted in erythroid commitment in megakaryocytic-erythroid progenitors, which was abrogated by knockdown of GATA1.  STAT5  is responsible for the immediate-early induction of the long isoform of the BCL2-related protein in erythroid cells through binding to the promoter of the BCLX gene.&nbsp

Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation. .

1. Friedrichsen, B. N., Galsgaard, E. D., Nielsen, J. H., Moldrup, A. Growth hormone- and prolactin-induced proliferation of insulinoma cells, INS-1, depends on activation of STAT5 (signal transducer and activator of transcription 5) . Molec. Endocr. 15: 136-148, 2001. 2. Hou, J., Schindler, U., Henzel, W. J., Wong, S. C., McKnight, S. L. Identification and purification of human Stat proteins activated in response to interleukin-2. Immunity 2: 321-329, 1995. 3. Schwaller, J., Parganas, E., Wang, D., Cain, D., Aster, J. C., Williams, I. R., Lee, C.-K., Gerthner, R., Kitamura, T., Frantsve, J., Anastasiadou, E., Loh, M. L., Levy, D. E., Ihle, J. N., Gilliland, D. G. Stat5 is essential for the myelo- and lymphoproliferative disease induced by TEL/JAK2.Molec. Cell 6: 693-704, 2000.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.