Anti-NOTCH4 Antibody Picoband® Fluoro488 Conjugated

NOTCH4 (NOTCH, DROSOPHILA, HOMOLOG OF, 4) also known as  INT3 or NOTCH3, FORMERLY, is a member of the Notch family. In situ hybridization revealed that  Notch4  transcripts are primarily restricted to endothelial cells in embryonic and adult life, suggesting a specific role forNotch4  during development of vertebrate endothelium. The sequences of the mouse and human  NOTCH4  proteins are 82% identical.  Northern blot analysis revealed that  NOTCH4 (S) is the major transcript and is expressed in a wide variety of tissues. Fluorescence in situ hybridization confirmed the location of the  NOTCH4  gene at chromosome 6p21.3. In linkage disequilibrium mapping of the MHC region in 80 British parent-offspring trios,   Wei and Hemmings (2000)   found that  NOTCH4  was highly associated with schizophrenia. Repression of  Notch4  resolved ataxia and reversed the disease progression, demonstrating that  Notch4  is not only sufficient to induce but also required to sustain the disease.&nbsp

Highly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. No expression was seen in adult brain or peripheral blood leukocytes.

Cardiogenesis, Cardiovascular, Epigenetics and Nuclear Signaling, Nuclear, Signaling Pathways, Stem Cells, Surface Molecules, Transcription, Transcription Factors/Regulators

Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system (By similarity) . .

1. Li, L., Huang, G. M., Banta, A. B., Deng, Y., Smith, T., Dong, P., Friedman, C., Chen, L., Trask, B. J., Spies, T., Rowen, L., Hood, L. Cloning, characterization, and the complete 56.8-kilobase DNA sequence of the human NOTCH4 gene. Genomics 51: 45-58, 1998. 2. Murphy, P. A., Lam, M. T. Y., Wu, X., Kim, T. N., Vartanian, S. M., Bollen, A. W., Carlson, T. R., Wang, R. A. Endothelial Notch4 signaling induces hallmarks of brain arteriovenous malformations in mice. Proc. Nat. Acad. Sci. 105: 10901-10906, 2008. 3. Wei, J., Hemmings, G. P. The NOTCH4 locus is associated with susceptibility to schizophrenia. (Letter) Nature Genet. 25: 376-377, 2000.