Anti-NME2 Antibody Picoband®

NME2 (NME/NM23 nucleoside diphosphate kinase 2) also called non-metastatic cells 2, protein (NM23B) expressed in, NM23-H2, NM23B or NDPKB, is identical to the beta subunit of human erythrocyte NDP kinase. Little to no expression was detected in other mouse tissues examined. Using Northern blot analysis,   Masse et al. (2002)   detected high expression of mouse  Nme2, which they called nm23-M2, in heart, liver, and kidney, with intermediate expression in skeletal muscle.  In situ hybridization of 15-day postcoitum mouse embryos showed ubiquitous  Nme2  expression. The mouse and human  NME2  genes contain 5 exons and span about 6.0 kb. The NME2 gene is mapped on 17q21.33. Srivastava et al. (2006)   concluded that histidine phosphorylation regulates KCa3.1 channel activity and that NDPBK is critical to the channel activity and the activation of CD4 T cells.

Nucleoside diphosphate kinase B; NDK B; NDP kinase B;2.7.4.6; C-myc purine-binding transcription factor PUF; Histidine protein kinase NDKB;2.7.13.3; nm23-H2; NME2; NM23B

Neurology Process, Neuroscience, Nucleotide Messenger, Oncoproteins/Suppressors, Pathways and Processes, Second Messenger, Signal Transduction, Tumor Suppressors

Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752) . Exhibits histidine protein kinase activity. .

1. Backer, J. M., Mendola, C. E., Kovesdi, I., Fairhurst, J. L., O'Hara, B., Eddy, R. L., Jr., Shows, T. B., Mathew, S., Murty, V. V. V. S., Chaganti, R. S. K. Chromosomal localization and nucleoside diphosphate kinase activity of human metastasis-suppressor genes NM23-1 and NM23-2.Oncogene 8: 497-502, 1993. 2. Masse, K., Dabernat, S., Bourbon, P.-M., Larou, M., Amrein, L., Barraud, P., Perel, Y., Camara, M., Landry, M., Lacombe, M.-L., Daniel, J.-Y.Characterization of the nm23-M2, nm23-M3 and nm23-M4 mouse genes: comparison with their human homologs. Gene 296: 87-97, 2002. 3. Srivastava, S., Li, Z., Ko, K., Choudhury, P., Albaqumi, M., Johnson, A. K., Yan, Y., Backer, J. M., Unutmaz, D., Coetzee, W. A., Skolnik, E. Y.Histidine phosphorylation of the potassium channel KCa3.1 by nucleoside diphosphate kinase B is required for activation of KCa3.1 and CD4 T cells. Molec. Cell 24: 665-675, 2006.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Cytoplasm. Nucleus. Cell projection, lamellipodium. Cell projection, ruffle. Isoform 2 is mainly cytoplasmic and isoform 1 and isoform 2 are excluded from the nucleolus. Colocalizes with ITGB1 and ITGB1BP1 at the edge or peripheral ruffles and lamellipodia during the early stages of cell spreading on fibronectin or collagen but not on vitronectin or laminin substrates.