Anti-Hyaluronidase-2 HYAL2 Antibody Picoband® Fluoro647 Conjugated

HYAL2 (hyaluronoglucosaminidase 2) also known as hyaluronidase 2, LuCa-2, is an  enzyme  that in humans is encoded by the  HYAL2  gene. HYAL2  cDNAs encode a preprotein with an N-terminal signal peptide.  Northern blot analysis indicated that  HYAL2  was expressed in all human tissues tested except adult brain, and Western blot analysis detected  Hyal2protein in all mouse tissues examined except adult brain. The HYAL2 gene is mapped on 3p21.31. HYAL2  is a glycosylphosphatidylinositol (GPI) -anchored protein on the cell surface and serves as a receptor for entry into the cell of the jaagsiekte sheep retrovirus (JSRV) . The findings of  Rai et al. (2001)   that  HYAL2  is a GPI-anchored protein on the cell surface showed that GFP would likely be cleaved from  HYAL2  during GPI addition, leaving  HYAL2  on the cell surface and resulting in GFP transit to the lysosome for degradation.

A synthetic peptide corresponding to a sequence at the C-terminus of human HYAL2, different from the related mouse and rat sequences by three amino acids.

Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product which is further hydrolyzed by sperm hyaluronidase to give small oligosaccharides. Displays very low levels of activity. Associates with and negatively regulates MST1R. .

1. Lepperdinger, G., Strobl, B., Kreil, G. HYAL2, a human gene expressed in many cells, encodes a lysosomal hyaluronidase with a novel type of specificity. J. Biol. Chem. 273: 22466-22470, 1998. 2. Rai, S. K., Duh, F.-M., Vigdorovich, V., Danilkovitch-Miagkova, A., Lerman, M. I., Miller, A. D. Candidate tumor suppressor HYAL2 is a glycosylphosphatidylinositol (GPI) -anchored cell-surface receptor for jaagsiekte sheep retrovirus, the envelope protein of which mediates oncogenic transformation. Proc. Nat. Acad. Sci. 98: 4443-4448, 2001. 3. Strobl, B., Wechselberger, C., Beier, D. R., Lepperdinger, G. Structural organization and chromosomal localization of Hyal2, a gene encoding a lysosomal hyaluronidase. Genomics 53: 214-219, 1998.