Anti-Transcription factor E2F6 E2F6 Antibody Picoband® Fluoro594 Conjugated

E2F6 (E2F transcription factor 6) also known as E2F-6. Northern blot analysis revealed that  E2F6  is expressed as 2 mRNAs, 2.5 and 3.5 kb, in all human tissues and cell lines tested. The predicted human and mouse  E2F6  protein sequences are 92% identical. The DNA-binding and dimerization domains of  E2F6  are highly related to those of other E2F family members, but this protein lacks the sequences necessary for either transcriptional activation or binding to RB1, p107, or p130. E2F6  can act to repress the transcription of E2F-responsive genes by countering the activity of other E2F complexes. E2F6  contributes to gene silencing in a manner independent of retinoblastoma protein family members. E2F6  is found in a multimeric protein complex that contains MGA and MAX, and thus the complex can bind not only to the E2F binding site but also to MYC- and Brachyury- binding sites.  The  E2F6  complex preferentially occupies target promoters in G0 cells rather than in G1 cells.

A synthetic peptide corresponding to a sequence in the middle region of human E2F6, different from the related mouse and rat sequences by one amino acid.

Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase.

1. Ogawa, H., Ishiguro, K., Gaubatz, S., Livingston, D. M., Nakatani, Y. A complex with chromatin modifiers that occupies E2F- and Myc-responsive genes in G0 cells. Science 296: 1132-1136, 2002. 2. Trimarchi, J. M., Fairchild, B., Verona, R., Moberg, K., Andon, N., Lees, J. A. E2F-6, a member of the E2F family that can behave as a transcriptional repressor. Proc. Nat. Acad. Sci. 95: 2850-2855, 1998.