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Anti-SSR3 Antibody Picoband®

Boster Bio Anti-SSR3 Antibody catalog # PA1796. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

SSR3 (signal sequence receptor, gamma) is also known as TRAPG. By immunoaffinity chromatography, biochemical purification, peptide sequence analysis, and screening of a rat liver cDNA library, Hartmann et al. (1993) obtained a cDNA encoding Ssr3, which they termed Trap-gamma. The deduced 185-amino acid protein spans the membrane 4 times and is expressed as a 20-kD protein. Gross (2012) mapped the SSR3 gene to chromosome 3q25.31 based on an alignment of the SSR3 sequence with the genomic sequence (GRCh37) . Hartmann et al. (1993) found that Ssr3 was associated in equal amounts with Ssr1, Ssr2, and Ssr4 in canine pancreatic microsomes.

Synonyms

Translocon-associated protein subunit gamma; TRAP-gamma; Signal sequence receptor subunit gamma; SSR-gamma; SSR3; TRAPG

Gene Name

SSR3

Gene ID

6747

UniProt

Q9UNL2

Host

Rabbit

Reactivity

Human, Monkey, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human SSR3, identical to the related rat and mouse sequences.

Clonality

Polyclonal

Tissue Specificity

Highly expressed in the brain, lung, kidney and heart. Expressed at low levels in the liver and spleen. .

Applications

IF, IHC, ICC, WB

Field of Research

Calcium Binding Proteins, Calcium Signaling, DNA/RNA, Epigenetics and Nuclear Signaling, ER Proteins, Organelle Proteins, Protein Trafficking, Signal Transduction, Signaling Pathway, Translation

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins.

References & Citations

1. Gross, M. B.Personal Communication.Baltimore, Md. 6/20/2012. 2. Hartmann, E., Gorlich, D., Kostka, S., Otto, A., Kraft, R., Knespel, S., Burger, E., Rapoport, T. A., Prehn, S.A tetrameric complex of membrane proteins in the endoplasmic reticulum.Europ. J. Biochem. 214: 375-381, 1993.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

21080 MW

Observed Molecular Weight

21 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Translocon-associated protein subunit gamma

Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein.

Protein Name

Translocon-associated protein subunit gamma (TRAP-gamma)

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

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