Anti-SNAP23 Antibody Picoband®

SNAP23 (Synaptosomal-Associated Protein, 23-KD), also called SNAP23A, is a protein that in humans is encoded by the SNAP23 gene. The SNAP23 gene has 8 exons, with the initiation codon located in exon 2. The SNAP23 gene is mapped on 15q15.1-q15.2. The SNAP23 cDNA encodes a 211-amino acid polypeptide with a predicted mass of 23 kD. Its amino acid sequence is 59% identical to that of SNAP25. Northern blot analysis revealed that SNAP23 is ubiquitously expressed. SNAP23 is able to bind to multiple syntaxins as well as to multiple vesicle-associated membrane proteins. After relocation, SNAP23 is required for exocytosis, implying a crucial role in promoting membrane fusion. TIVAMP-containing vesicles were concentrated in the apical domain of epithelial cells. STX3A and SNAP23 were codistributed at the apical plasma membrane, where they formed N-ethyl maleimide-dependent SNARE complexes with TIVAMP and cellubrevin. SNAP23 is structurally and functionally similar to SNAP25 and binds tightly to multiple syntaxins and synaptobrevins/VAMPs. It is an essential component of the high affinity receptor for the general membrane fusion machinery and is an important regulator of transport vesicle docking and fusion.

A synthetic peptide corresponding to a sequence at the C-terminus of human SNAP23, different from the related rat and mouse sequences by three amino acids.

Essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion.

1. Guo, Z., Turner, C., Castle, D. Relocation of the t-SNARE SNAP-23 from lamellipodia-like cell surface projections regulates compound exocytosis in mast cells. Cell 94: 537-548, 1998. 2. Lazo, P. A., Nadal, M., Ferrer, M., Area, E., Hernandez-Torres, J., Nabokina, S. M., Mollinedo, F., Estivill, X. Genomic organization, chromosomal localization, alternative splicing, and isoforms of the human synaptosome-associated protein-23 gene implicated in vesicle-membrane fusion processes. Hum. Genet. 108: 211-215, 2001. 3. Mollinedo, F., Lazo, P. A. Identification of two isoforms of the vesicle-membrane fusion protein SNAP-23 in human neutrophils and HL-60 cells. Biochem. Biophys. Res. Commun. 231: 808-812, 1997.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.