Anti-Replication factor C subunit 1 RFC1 Antibody Picoband® Fluoro488 Conjugated
Product Specifications
Background
RFC (Replication Factor C), also called activator-1, is a multimeric primer-recognition protein consisting of 5 distinct subunits of 145, 40, 38, 37, and 36.5 kD. Human RFC was purified from extracts of HeLa cells as a host factor essential for the in vitro replication of simian virus 40 (SV40) DNA. RFC, in the presence of ATP, assembles proliferating-cell nuclear antigen and DNA polymerase-delta or polymerase-epsilon on primed DNA templates. Tight interfacial coordination of the ATP analog ATP-gamma-S by RFC resulted in a spiral arrangement of the ATPase domains of the clamp loader above the PCNA ring.
Synonyms
Replication factor C subunit 1; Activator 1 140 kDa subunit; A1 140 kDa subunit; Activator 1 large subunit; Activator 1 subunit 1; DNA-binding protein PO-GA; Replication factor C 140 kDa subunit; RF-C 140 kDa subunit; RFC140; Replication factor C large subunit; RFC1; RFC140
Gene Name
RFC1
Gene ID
5981
UniProt
P35251
Host
Rabbit
Reactivity
Human
Cross Reactivity
No cross-reactivity with other proteins
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human RFC1.
Clonality
Polyclonal
Tissue Specificity
Wide tissue distribution. Undetectable in placental tissue.
Applications
Flow Cytometry
Field of Research
DNA/RNA, DNA Synthesis, Epigenetics and Nuclear Signaling
Purification
Immunogen affinity purified.
Form
Liquid
Function
The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA. .
References & Citations
1. Bermudez, V. P., Maniwa, Y., Tappin, I., Ozato, K., Yokomori, K., Hurwitz, J. The alternative Ctf18-Dcc1-Ctf8-replication factor C complex required for sister chromatid cohesion loads proliferating cell nuclear antigen onto DNA. Proc. Nat. Acad. Sci. 100: 10237-10242, 2003. 2. Peoples, R., Perez-Jurado, L., Wang, Y.-K., Kaplan, P., Francke, U. The gene for replication factor C subunit 2 (RFC2) is within the 7q11.23 Williams syndrome deletion. (Letter) Am. J. Hum. Genet. 58: 1370-1373, 1996.
Storage Conditions
At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Calculated Molecular Weight
128255 MW
Specificity
No cross reactivity with other proteins.
Applications Notes
6
Gene Name Synonym
Replication factor C subunit 1
Subcellular Location
Nucleus.
Sequence Similarities
Belongs to the activator 1 large subunit family.
Protein Name
Replication factor C subunit 1
Isotype
Rabbit IgG
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
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