Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

Anti-MCM2 Antibody Picoband®

Boster Bio Anti-MCM2 Antibody catalog # PA1711. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

MCM2 (MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 2), also known as MITOTIN, CDCL1 or BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division. And it is similar to members of the family of early S-phase proteins. The MCM2 gene is mapped to 3q21.3. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. In the G0 stage, the MCM2 and MCM5 proteins were much less abundant than the MCM7 and MCM3 proteins, which suggests that the MCM proteins are not present in stoichiometric amounts and that only a proportion of these molecules actively participate in cell cycle regulation as part of MCM complexes.

Synonyms

DNA replication licensing factor MCM2;3.6.4.12; Minichromosome maintenance protein 2 homolog; Nuclear protein BM28; Mcm2; Bm28, Cdcl1, Kiaa0030, Mcmd2

Gene Name

MCM2

Gene ID

17216

UniProt

P97310

Host

Rabbit

Reactivity

Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse MCM2, different from the related rat sequence by one amino acid.

Clonality

Polyclonal

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

DNA/RNA, DNA Synthesis, Epigenetics and Nuclear Signaling

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.

References & Citations

Blow, J. J., Laskey, R. A. A role for the nuclear envelope in controlling DNA replication within the cell cycle. Nature 332: 546-548, 1988. 2. Burkhart, R., Schulte, D., Hu, D., Musahl, C., Gohring, F., Knippers, R. Interactions of human nuclear proteins P1Mcm3 and P1Cdc46. Europ. J. Biochem. 228: 431-438, 1995. 3. Todorov IT, Lavigne J, Sakr F, Kaneva R, Foisy S, Bibor-Hardy V (Jul 1991) . Nuclear matrix protein mitotin messenger RNA is expressed at constant levels during the cell cycle. Biochem Biophys Res Commun 177 (1) : 395–400.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

102078 MW

Observed Molecular Weight

102 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

DNA replication licensing factor MCM2

Subcellular Location

Nucleus .

Sequence Similarities

Belongs to the MCM family.

Protein Name

DNA replication licensing factor MCM2

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

Mouse Anti-Pig IgM - AF594
BS21787-01 100 µL

Mouse Anti-Pig IgM - AF594

Ask
View Details
Mouse Anti-Pig IgM - AF594
BS21787-02 500 µL

Mouse Anti-Pig IgM - AF594

Ask
View Details
Kylt® Mycoplasma ovipneumoniae LD 25
31438 each

Kylt® Mycoplasma ovipneumoniae LD 25

Ask
View Details
Mouse Monoclonal FTO Antibody (FT62-6)
NBP2-80050-100ug 100 µg

Mouse Monoclonal FTO Antibody (FT62-6)

Ask
View Details
TAGLN Antibody
A36138-100UL 100 µL

TAGLN Antibody

Ask
View Details
Human Angiotensin I Converting Enzyme (ACE) ELISA Kit
abx050001-01 96 Tests

Human Angiotensin I Converting Enzyme (ACE) ELISA Kit

Ask
View Details