Anti-CYP11B1 Antibody Picoband®

CYP11B1 (Cytochrome p450, family 11, subfamily B, polypeptide 1), also called Steroid 11-beta-hydroxylase or P450C11, is a steroid hydroxylase found in the zona fasciculata. The CYP11B1 gene functions primarily in mitochondria in the zona fasciculata of the adrenal cortex to convert 11-deoxycortisol to cortisol and 11-deoxycorticosterone to corticosterone. CYP11B1 is a member of the cytochrome P450 superfamily of enzymes. It is mapped on 8q24.3. The CYP11B1 gene contains 9 exons and spans 6.5 kb. Using RT-PCR, Kayes-Wandover and White detected CYP11B1 mRNA in human cardiac tissue samples from left and right atria, aorta, apex, intraventricular septum, and atrioventricular node, as well as whole adult and fetal heart. Ventricles did not express CYP11B1. In patients with 11-beta-hydroxylase deficiency leading to hypertension and congenital adrenal hyperplasia, Pascoe et al. identified mutations in the CYP11B1 gene.

Cancer, Cancer Metabolism, Cardiovascular, Cytochromes, Endocrine Metabolism, Growth Factors/Hormones, Hormone Biosynthesis, Lipases, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism of Lipids and Lipoproteins, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Pathways and Processes, Signal Transduction

Has steroid 11-beta-hydroxylase activity. In addition to this activity, the 18 or 19-hydroxylation of steroids and the aromatization of androstendione to estrone have also been ascribed to cytochrome P450 XIB.

1. Chua, S. C., Szabo, P., Vitek, A., Grzeschik, K.-H., John, M., White, P. C. Cloning of cDNA encoding steroid 11-beta-hydroxylase (P450C11) . Proc. Nat. Acad. Sci. 84: 7193-7197, 1987. 2. Cicila, G. T., Rapp, J. P., Wang, J. M., St. Lezin, E., Ng, S. C., Kurtz, T. W. Linkage of 11-beta-hydroxylase mutations with altered steroid biosynthesis and blood pressure in the Dahl rat. Nature Genet. 3: 346-353, 1993. 3. Ezquieta, B., Luzuriaga, C. Neonatal salt-wasting and 11 beta-hydroxylase deficiency in a child carrying a homozygous deletion hybrid CYP11B2 (aldosterone synthase) -CYP11B1 (11 beta-hydroxylase) . Clin. Genet. 66: 229-235, 2004.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.