Anti-Eph receptor B3/EPHB3 Antibody Picoband®

Ephrin Receptor EphB3, is also known as human embryo kinase2 (HEK2) or Eph-like tyrosine kinase2 (ETK2) . HEK2, which is a member of the EPH/ELK family of tyrosine kinases, encodes a 998-amino acid polypeptide having a single putative transmembrane domain, a secretory signal sequence, and 2 fibronectin repeats. The EPHB3 gene is mapped to human chromosome 3q21-qter. HEK2 interacts with 2 ligands of EPH-related kinases (LERKs), namely, LERK2 (EFNB1) and LERK5 (EFNB2) . Coincubation of HEK2- and LERK2-expressing cells induces cell-cell adhesion and aggregation. Additionally, coexpression of HEK2 and LERK2 results in reduced kinase activity of HEK2.

Ephrin type-B receptor 3;2.7.10.1; EPH-like tyrosine kinase 2; EPH-like kinase 2; Embryonic kinase 2; EK2; hEK2; Tyrosine-protein kinase TYRO6; EPHB3; ETK2, HEK2, TYRO6

Angiogenesis, Axonal Guidance Proteins, Cancer, Cardiovascular, Cell Type Marker, Growth and Development, Intracellular Signaling, Kinases, Neurology Process, Neuron Marker, Neuroscience, Neurotransmission, Protein Phosphorylation, Receptor Tyrosine Kinases, Receptors / Channels, Signal Transduction, Tyrosine Kinases

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent biional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobes of the cerebral cortex. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt. .

1. Bohme, B., Holtrich, U., Wolf, G., Luzius, H., Grzeschik, K.-H., Strebhardt, K., Rubsamen-Waigmann, H.PCR mediated detection of a new human receptor-tyrosine-kinase, HEK 2.Oncogene 8: 2857-2862, 1993. 2. Bohme, B., VandenBos, T., Cerretti, D. P., Park, L. S., Holtrich, U., Rubsamen-Waigmann, H., Strebhardt, K.Cell-cell adhesion mediated by binding of membrane-anchored ligand LERK-2 to the EPH-related receptor human embryonal kinase 2 promotes tyrosine kinase activity.J. Biol. Chem. 271: 24747-24752, 1996.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.