Anti-Cullin 1/Cul1 Antibody Picoband® Fluoro594 Conjugated

Cullin 1, also known as CUL1, is a human protein and gene from cullin family. This protein plays an important role in protein degradation and protein ubiquitination. This is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. Human cullin 1, but not the other closely related cullins 2, 3, 4A, and 5, selectively interacts with human SKP1.

Core component of multiple cullin-RING-based SCF (SKP1- CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and exchange of the substrate recognition component is mediated by TIP120A/CAND1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF (BTRC) and SCF (FBXW11) ubiquitination of CTNNB1 and participate in Wnt signaling. SCF (FBXW11) s ubiquitination of phosphorylated NFKBIA. SCF (BTRC) s ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2. SCF (SKP2) s ubiquitination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF (SKP2) s ubiquitination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF (FBXW7) s ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF (FBXW2) s ubiquitination of GCM1. SCF (FBXO32) s ubiquitination of MYOD1. SCF (FBXO7) s ubiquitination of BIRC2 and DLGAP5. SCF (FBXO33) s ubiquitination of YBX1. SCF (FBXO1) s ubiquitination of BCL6 and DTL but does not seem to ubiquitination of TP53. SCF (BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF (CCNF) s ubiquitination of CCP110. SCF (FBXL3) and SCF (FBXL21) ubiquitination of CRY1 and CRY2. SCF (FBXO9) ubiquitination of TTI1 and TELO2. SCF (FBXO10) s ubiquitination of BCL2. .

1. Kipreos ET, Lander LE, Wing JP, et al. (1996) . cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family.. Cell 85 (6) : 829–39. Bonaldo MF, Lennon G, Soares MB (1997) . 2. Normalization and subtraction: two approaches to facilitate gene discovery.. Genome Res. 6 (9) : 791–806. 3. Michel, J. J., Xiong, Y. Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A. Cell Growth Differ. 9: 435-449, 1998.