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Anti-BCAT2 Antibody Picoband®

Boster Bio Anti-BCAT2 Antibody catalog # PA1502. Tested in Flow Cytometry, ICC/IF, IHC, WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

BCAT2, branched-chain aminotransferase 2, encodes a branched chain aminotransferase found in mitochondria. The encoded protein forms a dimer that catalyzes the first step in the production of the branched chain amino acids leucine, isoleucine, and valine. Multiple transcript variants encoding different isoforms have been found for this gene. The human BCAT2 gene is mapped to 19q13.

Synonyms

Branched-chain-amino-acid aminotransferase, mitochondrial; BCAT (m) ;2.6.1.42; Placental protein 18; PP18; BCAT2; BCATM, BCT2, ECA40

Gene Name

BCAT2

Gene ID

587

UniProt

O15382

Host

Rabbit

Reactivity

Human, Monkey

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human BCAT2.

Clonality

Polyclonal

Tissue Specificity

Ubiquitous.

Applications

Flow Cytometry, ICC/IF, IHC, WB

Field of Research

Amino Acid Metabolism, Amino Acids, Cancer, Metabolic Signaling Pathways, Metabolism, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids.

References & Citations

1. Yennawar NH, et al. Human mitochondrial branched chain aminotransferase isozyme: structural role of the CXXC center in catalysis. J Biol Chem, 2006 Dec 22. 2. Goto M, et al. Structural determinants for branched-chain aminotransferase isozyme-specific inhibition by the anticonvulsant drug gabapentin. J Biol Chem, 2005 Nov 4.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

44288 MW

Observed Molecular Weight

41 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Branched-chain-amino-acid aminotransferase, mitochondrial

Subcellular Location

Isoform A: Mitochondrion.

Sequence Similarities

Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.

Protein Name

Branched-chain-amino-acid aminotransferase, mitochondrial (BCAT (m) )

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

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