Anti-Interleukin-3 IL3 Antibody Picoband® Fluoro488 Conjugated

The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor (CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types.1 Interleukin-3 (IL-3), a protein of 140 amino acids, is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3.2 The cDNA sequence for murine interleukin-3, one of the colony stimulating factors that regulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide.3 The mouse IL 3 gene is located on chromosome 11.4 The human gene encoding IL 3 is tandemly arrayed on the long arm of chromosome 5.5 The standard product used in this kit is recombinant human IL-3, consisting of 133 amino acids with the molecular mass of 15KDa.

Interleukin-3; IL-3; Hematopoietic growth factor; Mast cell growth factor; MCGF; Multipotential colony-stimulating factor; P-cell-stimulating factor; IL3

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.

1. Le Beau, M. M.; Epstein, N. D.; O'Brien, S. J.; Nienhuis, A. W.; Yang, Y.-C.; Clark, S. C.; Rowley, J. D. The interleukin 3 gene is located on human chromosome 5 and is deleted in myeloid leukemias with a deletion of 5q. Proc. Nat. Acad. Sci. 84: 5913-5917, 1987. 2. Clark-Lewis, I.; Aebersold, R.; Ziltener, H.; Schrader, J. W.; Hood, L. E.; Kent, S. B. H. Automated chemical synthesis of a protein growth factor for hemopoietic cells, interleukin-3. Science 231: 134-139, 1986. 3. Fung, M. C.; Hapel, A. J.; Ymer, S.; Cohen, D. R.; Johnson, R. M.; Campbell, H. D.; Young, I. G. Molecular cloning of cDNA for murine interleukin-3. Nature 307: 233-237, 1984. 4. Ihle, J. N.; Silver, J.; Kozak, C. A. Genetic mapping of the mouse interleukin 3 gene to chromosome 11. J. Immun. 138: 3051-3054, 1987. 5. Yang, Y.-C.; Kovacic, S.; Kriz, R.; Wolf, S.; Clark, S. C.; Wellems, T. E.; Nienhuis, A.; Epstein, N. The human genes for GM-CSF and IL3 are closely linked in tandem on chromosome 5. Blood 71: 958-961, 1988.