Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.

Anti-Matrix metalloproteinase-16 MMP16 Antibody Picoband® Fluoro488 Conjugated

Q: What is the catalog number for Anti-Matrix metalloproteinase-16 MMP16 Antibody Picoband® Fluoro488 Conjugated?

The catalog number for Anti-Matrix metalloproteinase-16 MMP16 Antibody Picoband® Fluoro488 Conjugated is PA1123-Fluoro488.

Selected Variant

PA1123-Fluoro488

Size: 100 µg/Vial

Product Specifications

Background

The matrix metalloproteinase 16 (MMP16) protein consists of 604 amino acids and has a characteristic MMP domain structure, which gene is mapped on human chromosome 8q21. Additionally, MMP16 has a C-terminal extension containing a potential transmembrane domain, similar to MMP14 , MMP15 , and MMP17. Furthermore, it is membrane-bound and is a member of the membrane-type MMPs that are a subclass in the MMP family since the other members lack a C-terminal transmembrane domain and are secreted as soluble forms. MMP16 is expressed as a 12-kb transcript in brain, placenta, heart, and some carcinoma cell lines, but is not detectably expressed in lung, kidney, liver, spleen, and muscle.

Synonyms

Matrix metalloproteinase-16; MMP-16;3.4.24.-; MMP-X2; Membrane-type matrix metalloproteinase 3; MT-MMP 3; MTMMP3; Membrane-type-3 matrix metalloproteinase; MT3-MMP; MT3MMP; MMP16; MMPX2

Gene Name

MMP16

Gene ID

4325

UniProt

P51512

Host

Rabbit

Reactivity

Human, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human MMP16, identical to the related rat and mouse sequences.

Clonality

Polyclonal

Tissue Specificity

Expressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary.

Applications

Flow Cytometry

Field of Research

Invasion/Microenvironment, Metalloprotease, Proteolysis/Ubiquitin, Proteolytic Enzymes

Purification

Immunogen affinity purified.

Form

Liquid

Function

Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells. .

References & Citations

1. Sato, H.; Tanaka, M.; Takino, T.; Inoue, M.; Seiki, M. : Assignment of the human genes for membrane-type-1, -2, and -3 matrix metalloproteinases (MMP14, MMP15, and MMP16) to 14q12.2, 16q12.2-q21, and 8q21, respectively, by in situ hybridization. Genomics 39: 412-413, 1997. 2. Takino, T.; Sato, H.; Shinagawa, A.; Seiki, M. : Identification of the second membrane-type matrix metalloproteinase (MT-MMP-2) gene from a human placenta cDNA library: MT-MMPs form a unique membrane-type subclass in the MMP family. J. Biol. Chem. 270: 23013-23020, 1995.

Storage Conditions

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Calculated Molecular Weight

69521 MW

Specificity

No cross reactivity with other proteins.

Applications Notes

Gene Name Synonym

Matrix metalloproteinase-16

Subcellular Location

Isoform Long: Cell membrane ; Single-pass type I membrane protein ; Extracellular side . Localized at the cell surface of melanoma cells.

Sequence Similarities

Belongs to the peptidase M10A family.

Protein Name

Matrix metalloproteinase-16 (MMP-16)

Isotype

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

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