What sizes are available for Anti-Connexin 43/GJA1 Antibody Picoband® Fluoro647 Conjugated?

Anti-Connexin 43/GJA1 Antibody Picoband® Fluoro647 Conjugated is available in the following size(s): 100 µg/Vial.

How many freeze-thaw cycles can this product tolerate?

We recommend aliquoting the product upon receipt to avoid repeated freeze-thaw cycles, which can reduce activity and stability. This is your responsibility once the product has been delivered.

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.

Anti-Connexin 43/GJA1 Antibody Picoband® Fluoro647 Conjugated

Q: What is the catalog number for Anti-Connexin 43/GJA1 Antibody Picoband® Fluoro647 Conjugated?

The catalog number for Anti-Connexin 43/GJA1 Antibody Picoband® Fluoro647 Conjugated is PA1026-Fluoro647.

Product Specifications

Background

Connexins 43 (Cx43), also called GAP Junction Protein, alpha-1 (GJA1) . Connexin 43 is a member of the connexin gene family which abundantly expressed in the heart and liver and was mapped to 6q21-q23.2. Connexin43, the major protein of gap junctions in the heart, is targeted by several protein kinases that regulate myocardial cell-cell coupling. Mutations in the connexin43 gap-junction gene, which lead to abnormally regulated cell-cell communication, are associated with visceroatrial heterotaxia. Cx43 must also play a critical role in the physiology of hearing, presumably by participating in the recycling of potassium to the cochlear endolymph.

Synonyms

Gap junction alpha-1 protein; Connexin-43; Cx43; Gap junction 43 kDa heart protein; GJA1; GJAL

Gene Name

GJA1

Gene ID

2697

UniProt

P17302

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Connexin 43, identical to the related rat and mouse sequences.

Clonality

Polyclonal

Tissue Specificity

Expressed in the heart and fetal cochlea. .

Applications

Flow Cytometry

Field of Research

Atherosclerosis, Cardiovascular, Cell Adhesion, Cytoskeleton/ECM, Signal Transduction, Vascular Inflammation

Purification

Immunogen affinity purified.

Form

Liquid

Function

Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity) . .

References & Citations

1. Britz-Cunningham, S. H.; Shah, M. M.; Zuppan, C. W.; Fletcher, W. H. : Mutations of the connexin43 gap-junction gene in patients with heart malformations and defects of laterality. New Eng. J. Med. 332: 1323-1329, 1995. 2. Liu, X. Z.; Xia, X. J.; Adams, J.; Chen, Z. Y.; Welch, K. O.; Tekin, M.; Ouyang, X. M.; Kristiansen, A.; Pandya, A.; Balkany, T.; Arnos, K. S.; Nance, W. E. : Mutations in GJA1 (connexin 43) are associated with non-syndromic autosomal recessive deafness. Hum. Molec. Genet. 10: 2945-2951, 2001.

Storage Conditions

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Calculated Molecular Weight

43008 MW

Specificity

No cross reactivity with other proteins.

Applications Notes

Gene Name Synonym

Gap junction alpha-1 protein

Subcellular Location

Cell membrane ; Multi-pass membrane protein . Cell junction, gap junction . Endoplasmic reticulum .

Sequence Similarities

Belongs to the connexin family. Alpha-type (group II) subfamily.

Protein Name

Gap junction alpha-1 protein

Isotype

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

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