Anti-Apoptosis inhibitor 5/API5 Antibody Picoband® Fluoro488 Conjugated
Product Specifications
Background
Many growth factors and cytokines act as cellular survival factors by preventing programmed cell death (apoptosis) . Apoptosis inhibitor 5 (API5) is an antiapoptotic factor which may have a role in protein assembly. The API5 gene to chromosome 11p12 based on an alignment of the API5 sequence with the genomic sequence It is a critical determinant of E2F1-induced apoptosis, acting downstream of E2F to suppress E2F-dependent apoptosis without generally blocking E2F-dependent transcription.
Synonyms
Apoptosis inhibitor 5; API-5; Antiapoptosis clone 11 protein; AAC-11; Cell migration-inducing gene 8 protein; Fibroblast growth factor 2-interacting factor; FIF; Protein XAGL; API5; MIG8
Gene Name
API5
Gene ID
8539
UniProt
Q9BZZ5
Host
Rabbit
Reactivity
Human, Mouse, Rat
Cross Reactivity
No cross-reactivity with other proteins
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Apoptosis inhibitor 5, different from the related mouse sequence by one amino acid.
Clonality
Polyclonal
Tissue Specificity
Expressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in heart, pancreas and placenta. Highly expressed in several cancers. Preferentially expressed in squamous cell carcinoma versus adenocarcinoma in non-small cell lung cancer. .
Applications
Flow Cytometry
Field of Research
Apoptosis, Cancer, Cell Biology, Cell Death, Intracellular, Invasion/Microenvironment, Receptors
Purification
Immunogen affinity purified.
Form
Liquid
Function
Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. .
References & Citations
1. Hartz, P. A.Personal Communication.Baltimore, Md. 12/12/2005. 2. Tewari, M.; Yu, M.; Ross, B.; Dean, C.; Giordano, A.; Rubin, R. : AAC-11, a novel cDNA that inhibits apoptosis after growth factor withdrawal. Cancer Res. 57: 4063-4069, 1997.
Storage Conditions
At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Calculated Molecular Weight
59005 MW
Specificity
No cross reactivity with other proteins.
Applications Notes
6
Gene Name Synonym
Apoptosis inhibitor 5
Subcellular Location
Nucleus . Cytoplasm . Mainly nuclear. Can also be cytoplasmic.
Sequence Similarities
Belongs to the API5 family.
Protein Name
Apoptosis inhibitor 5 (API-5)
Isotype
Rabbit IgG
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
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