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Anti-Annexin IV/ANXA4 Antibody Picoband®

Boster Bio Anti-Annexin IV/ANXA4 Antibody catalog # PA1007-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Annexin IV, also known as placental anticoagulant protein II, or zymogen granule membrane-associated protein, 36-KD (ZAP36), is one of the family of calcium-dependent phospholipid-binding proteins whose normal function is uncertain. Annexin IV has 45 to 59% identity with other members of the annexin family. Annexin IV gene spans 18 to 56 kb. And it is located on mouse chromosome 6 in a region of conserved synteny with human chromosome 2. Rat annexin IV is ubiquitously expressed in epithelial tissues. Zap36 associated with zymogen granule membranes in dog and rat pancreas. Human annexin IV gene is mapped to chromosome 2p13.

Synonyms

Annexin A4;35-beta calcimedin; Annexin IV; Annexin-4; Carbohydrate-binding protein p33/p41; Chromobindin-4; Endonexin I; Lipocortin IV; P32.5; PP4-X; Placental anticoagulant protein II; PAP-II; Protein II; ANXA4; ANX4

Gene Name

ANXA4

Gene ID

307

UniProt

P09525

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Annexin IV, different from the related mouse and rat sequences by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Widely expressed, with highest expression in brain. .

Applications

IHC, WB

Field of Research

Calcium Binding Proteins, Calcium Signaling, Cardiovascular, Cell Adhesion, Cytoskeleton/ECM, Signal Transduction, Signaling Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. .

References & Citations

1. Barrow, L. L., Simin, K., Jones, J. M., Lee, D. C., Meisler, M. H. Conserved linkage of early growth response 4, annexin 4, and transforming growth factor alpha on mouse chromosome 6. Genomics 19: 388-390, 1994. 2. Fukuoka, S.-I., Kern, H., Kazuki-Sugino, R., Ikeda, Y. Cloning and characterization of ZAP36, an annexin-like, zymogen granule membrane associated protein, in exocrine pancreas. Biochim. Biophys. Acta 1575: 148-152, 2002. 3. Tait, J. F., Smith, C., Frankenberry, D. A., Miao, C. H., Adler, D. A., Disteche, C. M. Chromosomal mapping of the human annexin IV (ANX4) gene. Genomics 12: 313-318, 1992.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

35883 MW

Observed Molecular Weight

36 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Annexin A4

Subcellular Location

Nucleus . Cytoplasm . Cell projection, cilium . Basolateral cell membrane . Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium. Found in the cytoplasm of type II pneumocytes and alveolar macrophages. .

Sequence Similarities

Belongs to the annexin family.

Protein Name

Annexin A4

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

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