Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

Anti-Alkaline Phosphatase/ALPL Antibody Picoband®

Boster Bio Anti-Alkaline Phosphatase/ALPL Antibody catalog # PA1004-2. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Alkaline phosphatase (ALPL) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone) . Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. ALPL is the alkaline phosphatase of skin fibroblasts, the tissue-nonspecific type, and that it is active toward millimolar concentrations of the putative natural substrates phosphoethanolamine (PEA) and pyridoxal-5-prime-phosphate (PLP) . ALPL gene exists in single copy in the haploid genome and is composed of 12 exons distributed over more than 50 kb.Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.

Synonyms

Alkaline phosphatase, tissue-nonspecific isozyme; AP-TNAP; TNSALP;3.1.3.1 ; Alkaline phosphatase liver/bone/kidney isozyme; ALPL

Gene Name

ALPL

Gene ID

249

UniProt

P05186

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Alkaline Phosphatase, different from the related mouse and rat sequences by three amino acids.

Clonality

Polyclonal

Tissue Specificity

Widely expressed, with highest expression in brain. .

Applications

WB

Field of Research

Cell Biology, Cell Cycle, Kinases/Phosphatases, Tags & Cell Markers, Tumor Biomarkers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

This isozyme may play a role in skeletal mineralization.

References & Citations

1. Fedde, K. N.; Whyte, M. P. : Alkaline phosphatase (tissue-nonspecific isoenzyme) is a phosphoethanolamine and pyridoxal-5-prime-phosphate ectophosphatase: normal and hypophosphatasia fibroblast study. Am. J. Hum. Genet. 47: 767-775, 1990. 2. Weiss, M. J.; Cole, D. E. C.; Ray, K.; Whyte, M. P.; Lafferty, M. A.; Mulivor, R. A.; Harris, H. : A missense mutation in the human liver/bone/kidney alkaline phosphatase gene causing a lethal form of hypophosphatasia. Proc. Nat. Acad. Sci. 85: 7666-7669, 1988.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

57305 MW

Observed Molecular Weight

57 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Alkaline phosphatase, tissue-nonspecific isozyme

Subcellular Location

Cell membrane ; Lipid-anchor, GPI-anchor .

Sequence Similarities

Contains 12 fibronectin type-I domains.

Protein Name

Alkaline phosphatase, tissue-nonspecific isozyme

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

PIK3CG Antibody
A68935-100UL 100 µL

PIK3CG Antibody

Ask
View Details
Rabbit Monoclonal TOP2A Antibody (TOP2A/6570R) [DyLight 594]
NBP3-08757DL594 100 µL

Rabbit Monoclonal TOP2A Antibody (TOP2A/6570R) [DyLight 594]

Ask
View Details
FNTA Overexpression Lysate (Native) - (Dry Ice)
NBP2-08153 0.1 mg

FNTA Overexpression Lysate (Native) - (Dry Ice)

Ask
View Details
Hey2 (NM_013904) Mouse Tagged ORF Clone Lentiviral Particle
MR223872L3V 200 µL

Hey2 (NM_013904) Mouse Tagged ORF Clone Lentiviral Particle

Ask
View Details
5-Hydroxypyrimidine
279552-01 250 mg

5-Hydroxypyrimidine

Ask
View Details
5-Hydroxypyrimidine
279552-02 500 mg

5-Hydroxypyrimidine

Ask
View Details