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Anti-Gamma-Tubulin GGA3 Antibody (Monoclonal, GTU-88)

Selected Variant

MA1114

Size: 100 µg/Vial

Boster Bio Anti-Gamma-Tubulin GGA3 Antibody (Monoclonal, GTU-88) catalog # MA1114. Tested in ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Background

Gamma-tubulin is a universal component of microtubule organizing centers, which is essential for nuclear division and microtubule assembly in Aspergillus nidulans. TUBG1 mapped to within 20 kb of TUBG2 at 17q21. Gamma-tubulin is a minor protein, present at less than 1% the level of alpha- and beta-tubulin, and is limited to the centrosome. Gamma-tubulin is present in Drosophila melanogaster and Homo sapiens.

Synonyms

Golgi associated, gamma adaptin ear containing, ARF binding protein 3 (Predicted) ; Protein Gga3 ; Gga3 ; Gga3_predicted ; rCG_32821

Gene Name

GGA1; GGA2; GGA3

Gene ID

360658

UniProt

D3ZHG8

Host

Mouse

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Synthetic gamma-tubulin peptide (amino acids 38-53), conjugated to KLH.

Clonality

Monoclonal

Clone

Clone: GTU-88

Tissue Specificity

GGA1: Ubiquitously expressed.

Applications

ICC, WB

Field of Research

Golgi Proteins, Protein Trafficking, Signal Transduction

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

GGA1: Plays a role in protein sorting and trafficking betweenthe trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and bindsubiquitinated proteins and membrane cargo molecules with acytosolic acidic cluster-dileucine (AC-LL) motif.|GGA2: Plays a role in protein sorting and trafficking betweenthe trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and bindsubiquitinated proteins and membrane cargo molecules with acytosolic acidic cluster-dileucine (AC-LL) motif.|GGA3: Plays a role in protein sorting and trafficking betweenthe trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and bindsubiquitinated proteins and membrane cargo molecules with acytosolic acidic cluster-dileucine (AC-LL) motif.

References & Citations

1. Rommens, J. M.; Durocher, F.; McArthur, J.; Tonin, P.; LeBlanc, J.-F.; Allen, T.; Samson, C.; Ferri, L.; Narod, S.; Morgan, K.; Simard, J. : Generation of a transcription map at the HSD17B locus centromeric to BRCA1 at 17q21. Genomics 28: 530-542, 1995. 2. Zheng, Y.; Jung, M. K.; Oakley, B. R. : Gamma-tubulin is present in Drosophila melanogaster and Homo sapiens and is associated with the centrosome. Cell 65: 817-823, 1991. 3. Stearns, T.; Evans, L.; Kirschner, M. : Gamma-tubulin is a highly conserved component of the centrosome. Cell 65: 825-836, 1991.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

71715 MW

Observed Molecular Weight

55 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Golgi-associated, gamma adaptin ear containing, ARF binding protein 1; golgi-associated, gamma adaptin ear containing, ARF binding protein 2; golgi-associated, gamma adaptin ear containing, ARF binding protein 3

Subcellular Location

GGA1: Golgi apparatus, trans-Golgi networkmembrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein.|Gga1: Golgi apparatus, trans-Golgi networkmembrane Endosome membrane |GGA2: Golgi apparatus, trans-Golgi networkmembrane |Gga2: Golgi apparatus, trans-Golgi networkmembrane Endosome membrane |GGA3: Golgi apparatus, trans-Golgi networkmembrane |Gga3: Golgi apparatus, trans-Golgi networkmembrane Endosome membrane

Sequence Similarities

Belongs to the GGA protein family.

Protein Name

ADP-ribosylation factor-binding protein GGA1; ADP-ribosylation factor-binding protein GGA2; ADP-ribosylation factor-binding protein GGA3

Isotype

Mouse IgG1

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

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