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Anti-Myosin (Skeletal, Fast) MYH2 Antibody (Monoclonal, MY-32)

Boster Bio Anti-Myosin (Skeletal, Fast) MYH2 Antibody (Monoclonal, MY-32) catalog # MA1063. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rabbit, Rat.

Product Specifications

Background

Myosin is composed of 2 heavy chains of about 200,000 daltons each and 4 light chains of about 20,000 daltons each. The light chains are of 2 distinct types: the phosphorylatable, regulatory, or MLC2 type, and the nonphosphorylatable, alkali, or MLC1 and MLC3 types (MYL1 according to the HGM symbols) . Skeletal Myosin (Fast), , also known as MYL1, mapped to the region 2q32.1-qter.

Synonyms

Myh2 protein ; Myh2

Gene Name

MYH1; MYH2

Gene ID

Human (4619), Mouse (17879), Rat (287408), Human (4620), Mouse (17882), Rat (691644)

UniProt

I6L963

Host

Mouse

Reactivity

Human, Mouse, Rabbit, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Rabbit muscle myosin.

Clonality

Monoclonal

Clone

Clone: MY-32

Applications

IHC, WB

Field of Research

Cardiovascular, Contractility, Cytoskeleton, Cytoskeleton/ECM, Motor Proteins, Myosin, Signal Transduction

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

MYH1: Muscle contraction.|MYH2: Muscle contraction. Required for cytoskeletonorganization (By similarity) .

References & Citations

1. Cohen-Haguenauer, O.; Barton, P. J. R.; Van Cong, N.; Serero, S.; Gross, M.-S.; Jegou-Foubert, C.; de Tand, M.-F.; Robert, B.; Buckingham, M.; Frezal, J. : Assignment of the human fast skeletal muscle myosin alkali light chains gene (MLC1F/MLC3F) to 2q32.1-2qter. Hum. Genet. 78: 65-70, 1988. 2. Serero, S.; Barton, P.; Van Cong, N.; Cohen-Haguenauer, O.; Robert, B.; Buckingham, M.; Frezal, J. : Assignment of the human fast skeletal muscle myosin alkali light chains gene (MLC1F/MLC3F) to 2q32.1-2qter. (Abstract) Cytogenet. Cell Genet. 46: 690 only, 1987.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

183084 MW

Observed Molecular Weight

200 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Myosin, heavy chain 1, skeletal muscle, adult; myosin, heavy chain 2, skeletal muscle, adult

Subcellular Location

MYH1: Cytoplasm, myofibril. Note: Thick filamentsof the myofibrils.|Myh1: Cytoplasm, myofibril. Note: Thick filamentsof the myofibrils.|MYH2: Cytoplasm, myofibril. Note: Thick filamentsof the myofibrils.

Sequence Similarities

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.

Protein Name

Myosin-1; Myosin-2

Isotype

Mouse IgG1

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

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