Anti-Mcm7 Antibody (Monoclonal, DCS-141)

Initiation of DNA replication is a complex process involving the concerted action of many proteins. The chromosome 7-linked gene referred to by Nakatsuru et al. as MCM2 has been designated MCM7. The MCM2-7 complex is comprised of 6 subunits, MCM2 through MCM7, and is a ring-shaped heterohexameric ATPase involved in DNA replication. MCM7 is located in 7q21.3-q22.1.

MCM7: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once percell cycle' DNA replication initiation and elongation ineukaryotic cells. The active ATPase sites in the MCM2-7 ring areformed through the interaction surfaces of two neighboringsubunits such that a critical structure of a conserved argininefinger motif is provided in trans relative to the ATP-binding siteof the Walker A box of the adjacent subunit. The six ATPase activesites, however, are likely to contribute differentially to thecomplex helicase activity. Required for S-phase checkpointactivation upon UV-induced damage.

1. Nakatsuru, S.; Sudo, K.; Nakamura, Y. : Isolation and mapping of a human gene (MCM2) encoding a product homologous to yeast proteins involved in DNA replication. Cytogenet. Cell Genet. 68: 226-230, 1995. 2. Pacek, M.; Tutter, A. V.; Kubota, Y.; Takisawa, H.; Walter, J. C. : Localization of MCM2-7, Cdc45, and GINS to the site of DNA unwinding during eukaryotic DNA replication. Molec. Cell 21: 581-587, 2006.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.