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Anti-MAP1 Antibody (Monoclonal, HM-1)

Boster Bio Anti-MAP1 Antibody (Monoclonal, HM-1) catalog # MA1056. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat.

Product Specifications

Background

Microtubules are the ubiquitous cytoskeletal structural components that are involved in intracellular transport. They are composed of tubulin and microtubule-associated proteins (MAPs) . MAP1 is one of the major neuronal MAPs as well as being the largest (350KD) . MAPs include MAP1A, MAP1B, and MAP2. MAP1a is a single-copy gene spanning 10.5 kb. MAP1a coding sequence is contained in five exons. MAP1B is encoded as a polyprotein that is processed to form a complex N-terminal microtubule-binding domain.

Synonyms

Microtubule-associated protein 1A; MAP-1A; MAP1A heavy chain; MAP1 light chain LC2; Map1a; Mtap1a

Gene Name

MAP1A

Gene ID

Human (4130), Mouse (17754), Rat (25152)

UniProt

P34926

Host

Mouse

Reactivity

Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Rat brain microtubule-associated proteins (MAPs) .

Clonality

Monoclonal

Clone

Clone: HM-1

Tissue Specificity

Brain, heart and muscle.

Applications

IHC, WB

Field of Research

Cytoskeleton, Cytoskeleton/ECM, Microtubules, Neurogenesis, Neurology Process, Neuroscience, Signal Transduction

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

Structural protein involved in the filamentous cross- bridging between microtubules and other skeletal elements.

References & Citations

1. Fink, J. K.; Jones, S. M.; Esposito, C.; Wilkowski, J. : Human microtubule-associated protein 1a (MAP1A) gene: genomic organization, cDNA sequence, and developmental-and tissue-specific expression. Genomics 35: 577-585, 1996. 2. Ammarback, J. A.; Obar, R. A.; Hughes, S. M.; Vallee, R. B. : MAP1B is encoded as a polyprotein that is processed to form a complex N-terminal microtubule-binding domain. Neuron 7: 129-139, 1991.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

299531 MW

Observed Molecular Weight

42 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Microtubule-associated protein 1A

Subcellular Location

Cytoplasm, cytoskeleton .

Sequence Similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Protein Name

Microtubule-associated protein 1A

Isotype

Mouse IgG1

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

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