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Anti-Cytokeratin Peptide 18 KRT18 Antibody (Monoclonal, CY-90)

Boster Bio Anti-Cytokeratin Peptide 18 KRT18 Antibody (Monoclonal, CY-90) catalog # MA1026. Tested in IF, IHC, WB applications. This antibody reacts with Human.

Product Specifications

Background

Intermediate filaments (IFs) are a structurally related family of cellular proteins that appear to be intimately involved with the cytoskeleton. Human keratin 18 (KRT18) and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. The KRT18 gene is 3,791 bp long and the keratin 18 protein is coded for by 7 exons. The K18 gene is 3791 bp in length and the K18 protein is coded for by seven exons. By Southern blotting using the genomic DNA PCR product, the gene for keratin 18 is assigned to chromosome 12. Mutation of human keratin 18 in association with cryptogenic cirrhosis.

Synonyms

Keratin, type I cytoskeletal 18; Cytokeratin-18; CK-18; Keratin-18; K18; Krt18 ; Krt1-18

Gene Name

KRT18

Gene ID

Human (3875), Mouse (16668), Rat (294853)

UniProt

Q5BJY9

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

The human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines.

Clonality

Monoclonal

Clone

Clone: CY-90

Tissue Specificity

Expressed on the plasma membrane of hepatocytes and in the narrow apical portions of supporting cells in the vomeronasal sensory epithelium. Detected in the type III alveolar cells of the lung, in the proliferative crypt epithelium of the small intestine and in the older intragemmal cells of the tongue. .

Applications

IF, IHC, WB

Field of Research

Cell Type Markers, Class I, Cytoskeleton, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Intermediate Filaments, Microtubules, Signal Transduction, Structures, Tumor Associated

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6) -mediated barrier protection (By similarity) . Involved in the uptake of thrombin-antithrombin complexes by hepatic cells. .

References & Citations

1. Ku, N.-O.; Wright, T. L.; Terrault, N. A.; Gish, R.; Omary, M. B. : Mutation of human keratin 18 in association with cryptogenic cirrhosis. J. Clin. Invest. 99: 19-23, 1997. 2. Waseem, A.; Gough, A. C.; Spurr, N. K.; Lane, E. B. : Localization of the gene for human simple epithelial keratin 18 to chromosome 12 using polymerase chain reaction. Genomics 7: 188-194, 1990.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

47761 MW

Observed Molecular Weight

48 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Keratin, type I cytoskeletal 18

Subcellular Location

Nucleus matrix . Nucleus, nucleolus . Cytoplasm .

Sequence Similarities

Belongs to the intermediate filament family.

Protein Name

Keratin, type I cytoskeletal 18

Isotype

Mouse IgG1

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

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