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Anti-SHARPIN Antibody Picoband® APC Conjugated

Product Specifications

Background

Enables polyubiquitin modification-dependent protein binding activity. Involved in defense response to bacterium; protein linear polyubiquitination; and regulation of signal transduction. Located in cytosol. Part of LUBAC complex.

Synonyms

SHARPIN; SIPL1; PSEC0216; Sharpin; Shank-associated RH domain-interacting protein; Shank-interacting protein-like 1; hSIPL1

Gene Name

SHARPIN

Gene ID

81858

UniProt

Q9H0F6

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human SHARPIN recombinant protein (Position: H25-R325) . Human SHARPIN shares 72.3% and 72.6% amino acid (aa) sequence identity with mouse and rat SHARPIN, respectively.

Clonality

Polyclonal

Applications

Flow Cytometry

Purification

Immunogen affinity purified.

Form

Liquid

References & Citations

1. Ang, R. L., Chan, M., Legarda, D., Sundberg, J. P., Sun, S. C., Gillespie, V. L., Chun, N., Heeger, P. S., Xiong, H., Lira, S. A., Ting, A. T. Immune dysregulation in SHARPIN-deficient mice is dependent on CYLD-mediated cell death. Proc. Nat. Acad. Sci. 118: e2001602118, 2021. 2. Daigo, Y., Takayama, I., Ward, S. M., Sanders, K. M., Fujino, M. A. Novel human and mouse genes encoding a shank-interacting protein and its upregulation in gastric fundus of W/W (v) mouse. J. Gastroent. Hepatol. 18: 712-718, 2003. 3. Gerlach, B., Cordier, S. M., Schmukle, A. C., Emmerich, C. H., Rieser, E., Haas, T. L., Webb, A. I., Rickard, J. A., Anderton, H., Wong, W. W.-L., Nachbur, U., Gangoda, L., Warnken, U., Purcell, A. W., Silke, J., Walczak, H. Linear ubiquitination prevents inflammation and regulates immune signalling. Nature 471: 591-596, 2011.

Storage Conditions

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Calculated Molecular Weight

40 kDa

Applications Notes

6

Gene Name Synonym

SHANK associated RH domain interactor

Protein Name

Sharpin

Isotype

Rabbit IgG

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

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