Anti-RPL27 Antibody Picoband®

60S ribosomal protein L27 is a protein that in humans is encoded by the RPL27 gene. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L27e family of ribosomal proteins and a component of the 60S subunit. A splice site mutation in this gene has been identified in a Diamond-Blackfan anemia (DBA) patient. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

E.coli-derived human RPL27 recombinant protein (Position: M1-R108) . Human RPL27 shares 100% amino acid (aa) sequence identity with both mouse and rat RPL27.

1. Gallagher, R. A., McClean, P. M., Malik, A. N. Cloning and nucleotide sequence of a full length cDNA encoding ribosomal protein L27 from human fetal kidney. Biochim. Biophys. Acta 1217: 329-332, 1994. 2. Sim, E. U. H., Ang, C. H., Ng, C. C., Lee, C. W., Narayanan, K. Differential expression of a subset of ribosomal protein genes in cell lines derived from human nasopharyngeal epithelium. J. Hum. Genet. 55: 118-120, 2010. 3. Wang, R., Yoshida, K., Toki, T., Sawada, T., Uechi, T., Okuno, Y., Sato-Otsubo, A., Kudo, K., Kamimaki, I., Kanezaki, R., Shiraishi, Y., Chiba, K., and 21 others. Loss of function mutations in RPL27 and RPS27 identified by whole exome sequencing in Diamond-Blackfan anaemia. Brit. J. Haemat. 168: 854-864, 2015.

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.