Anti-NSUN4 Antibody Picoband®

NSUN4 belongsto a family of RNA 5-methylcytosine (m5C) methyltransferases (MTases) .MTERF4 recruits NSUN4 to the large ribosomal subunit in a process crucial for mitochondrial ribosome biogenesis. NSUN4 is a dual function protein, which on the one hand is needed for 12S rRNA methylation, and on the other hand interacts withMTERF4 to facilitate mitoribosomal assembly. The calculatedMWof NSUN4 is 43 kDa, 29786-1-AP can detect the band around 40 kDa.

1. Camara, Y., Asin-Cayuela, J., Park, C. B., Metodiev, M. D., Shi, Y., Ruzzenente, B., Kukat, C., Habermann, B., Wibom, R., Hultenby, K., Franz, T., Erdjument-Bromage, H., Tempst, P., Hallberg, B. M., Gustafsson, C. M., Larsson, N.-G. MTERF4 regulates translation by targeting the methyltransferase NSUN4 to the mammalian mitochondrial ribosome. Cell Metab. 13: 527-539, 2011. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 8/30/2013. 3. Spahr, H., Habermann, B., Gustafsson, C. M., Larsson, N.-G., Hallberg, B. M. Structure of the human MTERF4-NSUN4 protein complex that regulates mitochondrial ribosome biogenesis. Proc. Nat. Acad. Sci. 109: 15253-15258, 2012.

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.