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Anti-PHKG1 Antibody Picoband® Fluoro488 Conjugated

Product Specifications

Background

Phosphorylase b kinase gamma catalytic chain, skeletal muscle isoform is an enzyme that in humans is encoded by the PHKG1 gene. This gene is a member of the Ser/Thr protein kinase family and encodes a protein with one protein kinase domain and two calmodulin-binding domains. This protein is the catalytic member of a 16 subunit protein kinase complex which contains equimolar ratios of 4 subunit types. The complex is a crucial glycogenolytic regulatory enzyme. This gene has two pseudogenes at chromosome 7q11.21 and one at chromosome 11p11.12. Multiple transcript variants encoding different isoforms have been found for this gene.

Gene Name

PHKG1

Gene ID

5260

UniProt

Q16816

Host

Rabbit

Reactivity

Human

Immunogen

E.coli-derived human PHKG1 recombinant protein (Position: E62-R353) . Human PHKG1 shares 93.8% and 94.2% amino acid (aa) sequence identity with mouse and rat PHKG1, respectively.

Clonality

Polyclonal

Applications

Flow Cytometry

Purification

Immunogen affinity purified.

Form

Liquid

References & Citations

1. Burwinkel, B., Hu, B., Schroers, A., Clemens, P. R., Moses, S. W., Shin, Y. S., Pongratz, D., Vorgerd, M., Kilimann, M. W. Muscle glycogenosis with low phosphorylase kinase activity: mutations in PHKA1, PHKG1 or six other candidate genes explain only a minority of cases. Europ. J. Hum. Genet. 11: 516-526, 2003. 2. Chamberlain, J. S., vanTuinen, P., Reeves, A. A., Philip, B. A., Caskey, C. T. Isolation of cDNA clones for the catalytic gamma subunit of mouse muscle phosphorylase kinase: expression of mRNA in normal and mutant Phk mice. Proc. Nat. Acad. Sci. 84: 2886-2890, 1987. 3. Jones, T. A., da Cruz e Silva, E. F., Spurr, N. K., Sheer, D., Cohen, P. T. Localization of the gene encoding the catalytic gamma subunit of phosphorylase kinase to human chromosome bands 7p12-q21. Biochim. Biophys. Acta 1048: 24-29, 1990.

Storage Conditions

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Applications Notes

6

Isotype

IgG

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

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