Anti-PHKG1 Antibody Picoband®

Phosphorylase b kinase gamma catalytic chain, skeletal muscle isoform is an enzyme that in humans is encoded by the PHKG1 gene. This gene is a member of the Ser/Thr protein kinase family and encodes a protein with one protein kinase domain and two calmodulin-binding domains. This protein is the catalytic member of a 16 subunit protein kinase complex which contains equimolar ratios of 4 subunit types. The complex is a crucial glycogenolytic regulatory enzyme. This gene has two pseudogenes at chromosome 7q11.21 and one at chromosome 11p11.12. Multiple transcript variants encoding different isoforms have been found for this gene.

E.coli-derived human PHKG1 recombinant protein (Position: E62-R353) . Human PHKG1 shares 93.8% and 94.2% amino acid (aa) sequence identity with mouse and rat PHKG1, respectively.

1. Burwinkel, B., Hu, B., Schroers, A., Clemens, P. R., Moses, S. W., Shin, Y. S., Pongratz, D., Vorgerd, M., Kilimann, M. W. Muscle glycogenosis with low phosphorylase kinase activity: mutations in PHKA1, PHKG1 or six other candidate genes explain only a minority of cases. Europ. J. Hum. Genet. 11: 516-526, 2003. 2. Chamberlain, J. S., vanTuinen, P., Reeves, A. A., Philip, B. A., Caskey, C. T. Isolation of cDNA clones for the catalytic gamma subunit of mouse muscle phosphorylase kinase: expression of mRNA in normal and mutant Phk mice. Proc. Nat. Acad. Sci. 84: 2886-2890, 1987. 3. Jones, T. A., da Cruz e Silva, E. F., Spurr, N. K., Sheer, D., Cohen, P. T. Localization of the gene encoding the catalytic gamma subunit of phosphorylase kinase to human chromosome bands 7p12-q21. Biochim. Biophys. Acta 1048: 24-29, 1990.

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.