Anti-PSMC1 Antibody Picoband®

26S protease regulatory subunit 4, also known as 26S proteasome AAA-ATPase subunit Rpt2, is an enzyme that in humans is encoded by the PSMC1 gene. The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. This subunit and a 20S core alpha subunit interact specifically with the hepatitis B virus X protein, a protein critical to viral replication. This subunit also interacts with the adenovirus E1A protein and this interaction alters the activity of the proteasome. Finally, this subunit interacts with ataxin-7, suggesting a role for the proteasome in the development of spinocerebellar ataxia type 7, a progressive neurodegenerative disorder.

E.coli-derived human PSMC1 recombinant protein (Position: K21-L440) . Human PSMC1 shares 100% amino acid (aa) sequence identity with both mouse and rat PSMC1.

1. Aharoni, S., Proskorovski-Ohayon, R., Krishnan, R. K., Yogev, Y., Wormser, O., Hadar, N., Bakhrat, A., Alshafee, I., Gombosh, M., Agam, N., Gradstein, L., Shorer, Z., Zarivach, R., Eskin-Schwartz, M., Abdu, U., Birk, O. S. PSMC1 variant causes a novel neurological syndrome. Clin. Genet. 102: 324-332, 2022. 2. Dubiel, W., Ferrell, K., Pratt, G., Rechsteiner, M. Subunit 4 of the 26 S protease is a member of a novel eukaryotic ATPase family. J. Biol. Chem. 267: 22699-22702, 1992. 3. Gross, M. B. Personal Communication. Baltimore, Md. 2/17/2011.

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.