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Anti-POLD3 Antibody Picoband® Fluoro550 Conjugated

Product Specifications

Background

DNA polymerase delta subunit 3 is an enzyme that in humans is encoded by the POLD3 gene. This gene encodes the 66-kDa subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein plays a role in regulating the activity of DNA polymerase delta through interactions with other subunits and the processivity cofactor proliferating cell nuclear antigen (PCNA) . Alternatively spliced transcript variants have been observed for this gene.

Gene Name

POLD3

Gene ID

10714

UniProt

Q15054

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E.coli-derived human POLD3 recombinant protein (Position: D11-K296) . Human POLD3 shares 87.8% amino acid (aa) sequence identity with mouse POLD3.

Clonality

Polyclonal

Applications

Flow Cytometry

Purification

Immunogen affinity purified.

Form

Liquid

References & Citations

1. Dilley, R. L., Verma, P., Cho, N. W., Winters, H. D., Wondisford, A. R., Greenberg, R. A. Break-induced telomere synthesis underlies alternative telomere maintenance. Nature 539: 54-58, 2016. 2. Hughes, P., Tratner, I., Ducoux, M., Piard, K., Baldacci, G. Isolation and identification of the third subunit of mammalian DNA polymerase delta by PCNA-affinity chromatography of mouse FM3A cell extracts. Nucleic Acids Res. 27: 2108-2114, 1999. 3. Liu, G., Warbrick, E. The p66 and p12 subunits of DNA polymerase delta are modified by ubiquitin and ubiquitin-like proteins. Biochem. Biophys. Res. Commun. 349: 360-366, 2006.

Storage Conditions

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Applications Notes

6

Isotype

IgG

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

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