Anti-PLCE1 Antibody Picoband®

Phospholipase C epsilon 1 (PLCE1) is an enzyme that in humans is encoded by the PLCE1 gene. This gene encodes a phospholipase enzyme that catalyzes the hydrolysis of phosphatidylinositol-4,5-bisphosphate to generate two second messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) . These second messengers subsequently regulate various processes affecting cell growth, differentiation, and gene expression. This enzyme is regulated by small monomeric GTPases of the Ras and Rho families and by heterotrimeric G proteins. In addition to its phospholipase C catalytic activity, this enzyme has an N-terminal domain with guanine nucleotide exchange (GEF) activity. Mutations in this gene cause early-onset nephrotic syndrome; characterized by proteinuria, edema, and diffuse mesangial sclerosis or focal and segmental glomerulosclerosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

E.coli-derived human PLCE1 recombinant protein (Position: E4-E735) . Human PLCE1 shares 74.6% and 73.6% amino acid (aa) sequence identity with mouse and rat PLCE1, respectively.

1. Boyer, O., Benoit, G., Gribouval, O., Nevo, F., Pawtowski, A., Bilge, I., Bircan, Z., Deschenes, G., Guay-Woodford, L. M., Hall, M., Macher, M.-A., Soulami, K., Stefanidis, C. J., Weiss, R., Loirat, C., Gubler, M.-C., Antignac, C. Mutational analysis of the PLCE1 gene in steroid resistant nephrotic syndrome. J. Med. Genet. 47: 445-452, 2010. 2. Bunney, T. D., Harris, R., Lamuno Gandarillas, N., Josephs, M. B., Roe, S. M., Sorli, S. C., Paterson, H. F., Rodrigues-Lima, F., Esposito, D., Ponting, C. P., Gierschik, P., Pearl, L. H., Driscoll, P. C., Katan, M. Structural and mechanistic insights into Ras association domains of phospholipase C epsilon. Molec. Cell 21: 495-507, 2006. 3. Evellin, S., Nolte, J., Tysack, K., vom Dorp, F., Thiel, M., Oude Weernink, P. A., Jakobs, K. H., Webb, E. J., Lomasney, J. W., Schmidt, M. Stimulation of phospholipase C-epsilon by the M (3) muscarinic acetylcholine receptor mediated by cyclic AMP and the GTPase Rap2B. J. Biol. Chem. 277: 16805-16813, 2002.

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.