Anti-PIK3R4 Antibody Picoband® Fluoro594 Conjugated

Phosphoinositide 3-kinase regulatory subunit 4, also known as PI3-kinase regulatory subunit 4 or PI3-kinase p150 subunit or phosphoinositide 3-kinase adaptor protein, or VPS15 is an enzyme that in humans is encoded by the PIK3R4 gene. The serine/threonine PI3 kinase regulatory subunit 4 (PIK3R4, Vps15) is the mammalian homologue of the yeast vacuolar protein sorting 15. PIK3R4 regulates the kinase activity of PI3K class III and anchors the kinase to cellular membranes through myristoylation. Recruitment of PI3K class III to the site of early endosome fusion and docking is ly mediated by PIK3R4 binding to the small GTPase Rab5 through its HEAT and WD-40 domains. The PIK3R4/PI3K class III plays a role in late endosome function through PIK3R4 binding to the Rab7 GTPase. In addition to its role in trafficking, the PIK3R4/PI3K class III complex interacts with beclin-1 to play a role during several stages of autophagy. Autophagosome formation is stimulated when Atg14 complexes with PIK3R4, PI3K class III, and beclin-1. The UVRAG protein competes with Atg14 for beclin-1 binding, forming a mutually exclusive complex with PIK3R4, PI3K class III, and beclin-1 that regulates autophagosome maturation. Autophagosome maturation is impaired in the presence of the beclin-1-binding protein Rubicon. Co-expression of PIK3R4 is required for PI3K class III activation and regulation by both beclin-1/UVRAG and by nutrients. Overexpression of PIK3R4 protein has been associated with decreased survival in patients with ovarian tumors, while mutations of the corresponding PIK3R4 gene are associated with metastatic melanoma, suggesting that PIK3R4 functions in cancer.

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E.coli-derived human PIK3R4 recombinant protein (Position: R36-Q1339) . Human PIK3R4 shares 95.7% and 95.6% amino acid (aa) sequence identity with mouse and rat PIK3R4, respectively.

Downstream effector of TOR signaling pathway involved in osmotic stress response. Could be involved in the control of plant growth and development. Phosphorylates the ribosomal proteins P14, P16 and S6. Functions as a repressor of cell proliferation and required for maintenance of chromosome stability and ploidy levels through the RBR1-E2F pathway.

1. Gross, M. B. Personal Communication. Baltimore, Md. 5/16/2018. 2. Gstrein, T., Edwards, A., Pristoupilova, A., Leca, I., Breuss, M., Pilat-Carotta, S., Hansen, A. H., Tripathy, R., Traunbauer, A. K., Hochstoeger, T., Rosoklija, G., Repic, M., and 15 others. Mutations in Vps15 perturb neuronal migration in mice and are associated with neurodevelopmental disease in humans. Nature Neurosci. 21: 207-217, 2018. Note: Erratum: Nature Neurosci. 21: 1139 only, 2018. 3. Panaretou, C., Domin, J., Cockcroft, S., Waterfield, M. D. Characterization of p150, an adaptor protein for the human phosphatidylinositol (PtdIns) 3-kinase: substrate presentation by phosphatidylinositol transfer protein to the p150-PtdIns 3-kinase complex. J. Biol. Chem. 272: 2477-2485, 1997.