Anti-MELK Antibody Picoband® Fluoro647 Conjugated
Product Specifications
Background
Maternal embryonic leucine zipper kinase (MELK) is an enzyme that in humans is encoded by the MELK gene. Maternal embryonic leucine-zipper kinase (MELK) is a key regulator of survival of stemlike GBM cells in vitro. MELK expression is increased in breast cancer tissue and this increase is also associated with poor patient survival, as predicted for a candidate oncogene.
Synonyms
Homeobox protein Hox-A5; Homeobox protein Hox-1C; HOXA5; HOX1C
Gene Name
MELK
Gene ID
9833
UniProt
Q14680
Host
Rabbit
Reactivity
Human, Mouse, Rat
Cross Reactivity
No cross-reactivity with other proteins.
Immunogen
E.coli-derived human MELK recombinant protein (Position: H66-Q629) .
Clonality
Polyclonal
Tissue Specificity
Ubiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. In the kidney, it is widely expressed in tubules, but sparsely expressed in the glomerulus (PubMed:24676636) . Expression is significantly increased in renal biopsy specimens from idiopathic FSGS (PubMed:24676636) . Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors.
Applications
Flow Cytometry
Field of Research
Cancer, Cell Cycle, Cell Cycle Inhibitors, Developmental Families, Domain Families, Epigenetics and Nuclear Signaling, p53 Pathway, Transcription
Purification
Immunogen affinity purified.
Form
Liquid
Function
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Also binds to its own promoter. Binds specifically to the motif 5'-CYYNATTA[TG]Y-3'.
References & Citations
1. Heyer, B. S., Warsowe, J., Solter, D., Knowles, B. B., Ackerman, S. L. New member of the Snf1/AMPK kinase family, Melk, is expressed in the mouse egg and preimplantation embryo. Molec. Reprod. Dev. 47: 148-156, 1997. 2. Hirose, T., Horvitz, H. R. An Sp1 transcription factor coordinates caspase-dependent and -independent apoptotic pathways. Nature 500: 354-358, 2013. 3. Nagase, T., Seki, N., Ishikawa, K., Tanaka, A., Nomura, N. Prediction of the coding sequences of unidentified human genes. V. The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by analysis of cDNA clones from human cell line KG-1. DNA Res. 3: 17-24, 1996.
Storage Conditions
At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Calculated Molecular Weight
23310 MW
Applications Notes
6
Gene Name Synonym
Homeobox A5
Subcellular Location
Nucleus.
Protein Name
Ran-specific GTPase-activating protein
Isotype
Rabbit IgG
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
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